贵州医科大学学报

2014, v.39;No.171(06) 820-824

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

APC基因在宫颈癌及其癌前病变中的表达及相关性
Expression of APC Genes in Cervical Cancer and Precancerous Lesion and Its Correlation with Development of Cervical Cancer

訾聃;任念;杨英捷;
ZI Dan;REN Nian;YANG Yingjie;Affiliated Hospital of Guiyang Medical University;Guizhou Provincial Tumor Hospital;

摘要(Abstract):

目的:探讨APC基因在HPV16感染的宫颈癌及其癌前病变中的表达情况及相关性。方法:将接受手术的33例宫颈癌患者和28例宫颈上皮内瘤变(CINⅢ)患者分为宫颈癌组和CINⅢ组,同期手术的31例子宫肌瘤或子宫腺肌症患者作为对照组;3组患者术前均行宫颈细胞凯普导流杂交人乳头瘤病毒DNA(HPV DNA)检测HPV16,计算HPV16 DNA阳性表达率;RT-PCR及免疫组化方法检测APC mRNA及APC蛋白的表达,分析宫颈癌及CINⅢ组中HPV16 DNA阳性率与APC mRNA及蛋白表达的相关性。结果:宫颈癌组与CIN III组的HPV16 DNA阳性率明显高于对照组,差异有统计学意义(P<0.05);宫颈癌组APC mRNA及蛋白的阳性表达率低于照组,差异有统计学意义(P<0.05);CINⅢ组APC mRNA表达率与对照组差异无统计学意义(P>0.05);宫颈癌及CINⅢ组中HPV16 DNA阳性率与APC mRNA及蛋白的表达呈负相关性(r=-0.573,-0.616;r=-0.573,-0.654;P<0.05)。结论:APC基因与HPV16可能共同参与了宫颈由正常至CIN进而发展为宫鳞癌的恶性转变过程。
Objective: To investigate the expression of APC genes in HPV16 infected cervical cancer and precancerous lesions,and to explore its correlation with development of cervical cancer. Methods: Thirty-three cervical cancer patients and 28 cervical intraepithelial neoplasia( CINIII) patients all received operation,they were divided into cervical cancer group and CINIII group; 31 hysteromyoma or adenomyosis patients served as control group; HPV16 of all three group patients were tested by cervical cell CAPE diversion hybridization instrument before surgery,it aimed to calculate the positive rate of HPV16 DNA; APC mRNA and protein were tested by RT-PCR and immunohistochemistry,in order to analyze correlation of HPV16 DNA positive rate in cervical cancer group and CINIII group with APC mRNA and protein expression. Results: The positive rates of HPV16 DNA in cervical cancer group and CIN III group were obviously higher than those of control group,the differences were statistically significant( P < 0. 05),while the positive rates of APC mRNA and protein of the two groups were lower than those of the control group,the differences were statistically significant( P < 0. 05); the positive rates of APC mRNA in CIN III group and control group had no significant difference( P > 0. 05).HPV16 DNA positive rates of in cancer group and CIN III group were negatively correlated with APC mRNA and protein expression( r =- 0. 573,- 0. 616; r =- 0. 573,- 0. 654; P < 0. 05). Conclusions: Both APC gene and HPV16 may act synergistically in development and progression of cervical cancer.

关键词(KeyWords): 宫颈肿瘤;宫颈上皮内瘤样病变;APC基因;人乳头瘤病毒;DNA,病毒
cervical cancer;cervical intraepithelial neoplasia;APC gene;human papillomavirus;DNA,virus

Abstract:

Keywords:

基金项目(Foundation): 贵州省科学技术厅合同编号[黔科合J字(2009)2211];; 贵阳市科技局项目合同编号[筑科合同(2012103)31号]

作者(Author): 訾聃;任念;杨英捷;
ZI Dan;REN Nian;YANG Yingjie;Affiliated Hospital of Guiyang Medical University;Guizhou Provincial Tumor Hospital;

Email:

DOI:

参考文献(References):

文章评论(Comment):

序号(No.) 时间(Time) 反馈人(User) 邮箱(Email) 标题(Title) 内容(Content)
反馈人(User) 邮箱地址(Email)
反馈标题(Title)
反馈内容(Content)
扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享