贵州医科大学学报

2016, v.41;No.185(02) 179-181

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烧伤早期注射胰岛素对大鼠心、肾损害的影响
Effect of Hypodemic Injection of Protamin Biosynthetic Insulin on the Damage of Heart and Kidney of Delayed Resuscitation Rats in the Early Stage of Burn Injury

袁新章;王伟;李伟人;
YUAN Xinzhang;WANG Wei;LI Weiren;Department of Burn and Plastic Surgery,the Affiliated Hospital of Guizhou Medical University;

摘要(Abstract):

目的:探讨烧伤早期皮下注射精蛋白生物合成胰岛素对延迟复苏大鼠心、肾损害的影响。方法:32只SPF级SD大鼠随机均分为假伤组、即时复苏组、延迟复苏组与延迟复苏治疗组4组;即时复苏组、延迟复苏组与延迟复苏治疗组大鼠置于(95.0±0.5)℃热水中15 s,制作30%烧伤总面积(TBSA)的Ⅲ°烫伤动物模型,假伤组置37℃温水中15 s模拟烫伤;即时复苏组和延迟复苏组伤后立即腹腔注射生理盐水(40 m L/kg),延迟复苏治疗组伤后立即在右后肢腹侧面皮下注射精蛋白生物合成胰岛素(2.0 U/kg),延迟复苏组与延迟复苏治疗组伤后6 h时腹腔注射生理盐水(40 m L/kg);各组均于伤后24 h时采集腹主动脉血,采用分光光度法测定血糖(BG)、乳酸脱氢酶(LDH)、肌酸激酶(CK)、尿素氮(BUN)及肌酐(Cr)。结果:即时复苏组、延迟复苏组与延迟复苏治疗组BG、LDH、CK、BUN、Cr均显著高于假伤组(P<0.05),延迟复苏组BG、LDH、CK、BUN、Cr显著高于即时复苏组(P<0.05),延迟复苏治疗组BG、LDH、CK、BUN、Cr显著低于延迟复苏组(P<0.05),延迟复苏治疗组LDH、CK显著高于即时复苏组(P<0.05),延迟复苏治疗组BG、Cr显著低于即时复苏组(P<0.05),延迟复苏治疗组BUN与即时复苏组比较无显著差异(P>0.05)。结论:烧伤早期皮下注射精蛋白生物合成胰岛素可减轻延迟复苏大鼠的心脏及肾脏损害。
Objective: To investigate the effect of hypodemic injection of protamin biosynthetic insulin on the damage of hearts and kidneys of rats with delayed resuscitation in the early stage of burn injury. Method: Thirty-two specific-pathogen free Sprague-Dawley rats were randomly divided into 4groups,namely,sham burn group,immediate resuscitation group,delayed resuscitation group and delayed resuscitation treatment group,with 8 rats in each group. The rats of immediate resuscitation group,delayed resuscitation group and delayed resuscitation treatment group were immersed in(95 ±0. 5) ℃ hot water for 15 seconds to make empyrosis rat model of 30% total burn surface area,Ⅲ degree burn injury. The sham burn group was immersed in 37 ℃ warm water for 15 seconds to simulate burns. Immediate resuscitation group and delayed resuscitation group received an intraperitoneal injection of physiological saline( 40 m L / kg) after burn injury immediately,while delayed resuscitation treatment group received subcutaneous injection of protamine biosynthetic insulin{(2. 0 U / kg) in the ventral side of the right hind limb after burn injury immediately. Delayed resuscitation group and delayed resuscitation treatment group received intraperitoneal injection of physiological saline( 40 m L /kg) 6 hours after burn injury. The abdominal aortic blood was collected 24 hours after burn injury in each group. The spectrophotometry was adopted for determination of blood glucose( BG),lactate dehydrogenase( LDH),creatine kinase( CK),blood urea nitrogen( BUN) and creatinine( Cr). Results:BG,LDH,CK,BUN and Cr in immediate resuscitation group,delayed resuscitation group and delayed resuscitation treatment group were significantly higher than their counterparts of sham burn group(P < 0. 05). BG,LDH,CK,BUN and Cr in delayed resuscitation group were totally higher than their counterparts of immediate resuscitation group( P < 0. 05). BG,LDH,CK,BUN and Cr in delayed resuscitation treatment group were significantly lower than their counterparts of delayed resuscitation group( P < 0. 05). LDH and CK in delayed resuscitation treatment group were significantly higher than their counterparts of immediate resuscitation group,while BG and Cr were significantly lower than their counterparts of immediate resuscitation group( P < 0. 05). There was no significant difference of BUN between delayed resuscitation treatment group and immediate resuscitation group( P > 0. 05).Conclusion: During the early stage of burn injury,hypodemic injection of protamine biosynthetic insulin can reduce the damage of heart and kidney of delayed resuscitation rats.

关键词(KeyWords): 烧伤;复苏;胰岛素;心脏;肾
burn;recovery;insulin;heart;kidney

Abstract:

Keywords:

基金项目(Foundation): 贵州省卫生厅科学技术基金(gzwkj2011-1-022)

作者(Author): 袁新章;王伟;李伟人;
YUAN Xinzhang;WANG Wei;LI Weiren;Department of Burn and Plastic Surgery,the Affiliated Hospital of Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2016.02.015

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