贵州医科大学学报

2017, v.42;No.201(06) 650-654+660

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洛伐他汀对抗AβOs引起的SH-SY5Y细胞毒性作用
Antagonism of Lovastatin on Cytotoxicity in SH-SY5Y Cells Induced by β-Amyloid Peptide Oligomers

谭龙春;吴昌学;赵亮;董阳婷;刘仙红;官志忠;
TAN Longchun;WU Changxue;ZHAO Liang;DONG Yangting;LIU Xianhong;GUAN Zhizhong;The Key Laboratory of Molecular Biology,Guizhou Medical University;The Key Laboratory of Endemic and Ethnic Diseases,Guizhou Medical University,Ministry of Education of P.R.China;Department of Microbiology,Guizhou Medical University;Department of Pathology,the Affiliated Hospital of Guizhou Medical University;

摘要(Abstract):

目的:观察洛伐他汀对抗Aβ寡聚体(AβOs)引起的SH-SY5Y细胞毒性作用。方法:生长至80%~90%并用无血清培养基培养12 h的SH-SY5Y细胞分为对照组、洛伐他汀处理组(0.1μmol/L洛伐他汀处理24 h)、AβOs处理组(0.5μmol/L AβOs处理48 h)和洛伐他汀加AβOs处理组(0.1μmol/L洛伐他汀处理24 h,再加入0.5μmol/L AβOs继续处理48 h),采用CCK-8法检测各组细胞活性,使用相应的试剂盒检测细胞超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MDA)含量。结果:0.5μmol/L AβOs处理SHSY5Y细胞48 h后,与对照组相比,SH-SY5Y细胞活性、SOD及GSH-Px活性显著降低,MDA含量明显增加(P<0.05);在0.5μmol/L AβOs处理SH-SY5Y细胞前,预先用0.1μmol/L洛伐他汀处理细胞24 h,可减弱AβOs引起的细胞活性、SOD和GSH-Px活性及MDA含量的改变(P<0.05)。结论:洛伐他汀可对抗AβOs引起的SHSY5Y细胞毒性作用,其保护机制可能与抑制氧化应激有关。
Objective: To investigate the effect of lovastatin on cytotoxicity in SH-SY5 Y cells induced by β-Amyloid peptide oligomers( AβOs). Methods: When SH-SY5 Y cells were grown to 80% ~90% and cultured in serum-free medium for 12 h,they were divided into control group,lovastatin group( 0. 1 μmol/L lovastatin for 24 h),AβOs group( 0. 5 μmol/L βOs for 48 h) and lovastatin +AβOs group( 0. 1 μmol/L lovastatin treated with for 24 h,and then treated with 0. 5 μmol/L AβOs for 48 h). The cell viability was measured by CCK-8 assay,superoxide dismutase( SOD) and glutathione peroxidase( GSH-PX) activities and malondialdehyde( MDA) content were measured by appropriate biochemical methods. Results: As compared with control group,the cell viability,SOD and GSH-PX activities were significant decreased,while MDA content was significantly increased after SHSY5 Y cells treated with 0. 5 μmol/L AβOs for 48 h( P < 0. 05). When SH-SY5 Y cells were treatedwith 0. 1 μmol/L lovastatin for 24 h before treatments of AβOs,these cytotoxicity resulted by AβOs indicated as cell viability,SOD and GSH-PX activity and MDA content were all significantly attenuated( P < 0. 05). Conclusions: Lovastatin could antagonize the cytotoxicity in SH-SY5 Y cells caused by AβOs,and the mechanism might relate to the inhibition of oxidative stress.

关键词(KeyWords): 阿尔茨海默病;洛伐他汀;Aβ寡聚体;细胞毒性;氧化应激;SH-SY5Y细胞
Alzheimer disease;lovastatin;β-amyloid peptide oligomers;cytotoxicity;oxidative stress;SH-SY5Y cells

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81260173);; 教育部“长江学者和创新团队发展计划资助”(IRT13058);; 贵州省科技计划[黔科合重大专项字(2014)6008号];; 贵州省创新计划项目[黔教合协同创新中心(2014)06]

作者(Author): 谭龙春;吴昌学;赵亮;董阳婷;刘仙红;官志忠;
TAN Longchun;WU Changxue;ZHAO Liang;DONG Yangting;LIU Xianhong;GUAN Zhizhong;The Key Laboratory of Molecular Biology,Guizhou Medical University;The Key Laboratory of Endemic and Ethnic Diseases,Guizhou Medical University,Ministry of Education of P.R.China;Department of Microbiology,Guizhou Medical University;Department of Pathology,the Affiliated Hospital of Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2017.06.007

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