贵州医科大学学报

2002, (06) 509-510+513

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血管紧张素转化酶基因多态性与血清酶水平及动力学常数的研究
An Analysis of Angiotensin Converting Enzyme Gene Polymorphism with its Activity Levels and Kinetics Constants in the Serum

黄海,陶建蜀,禄永汉
HUANG Hai, TAO Jian shu, LU Yong han (The Department of Clinical Biochemistry, the Medical Laboratory Sciences Faculty of Guiyang Medical College, Guiyang, China 550004)

摘要(Abstract):

目的 :为探讨血管紧张素转化酶 (ACE)I/D多态性影响血清ACE活力水平的可能机制。方法 :采用紫外比色法 ,以马尿酰甘氨酰甘氨酸为底物测定ACE活力和动力学常数 ,以PCR检测I/D多态性。结果 :4 5 %的活力水平改变由I/D多态性决定 ,DD、ID、II三种基因型之间的Km值无明显差异 ,Vmax则因I/D而异 ,依次为DD >ID >II。结论 :血清ACE水平与其I/D基因多态性有关 ,该多态性没有影响到酶与底物HGG的亲活性。
Objective:To investigate the possible mechanism that ACE I/D plymorphism induces the different levels of ACE activity in the serum. Methods: PCR was used to detect ACE I/D polymorphism and Hippurylglysylglycine(HGG)was used as substrate to test the serum ACE activity and kinetics constants. Results: 45% of ACE activity variance were modulated by I/D polymorphism. The Michaelis-Menten constants of ACE were not different from DD, ID or Ⅱ(p>0.05). Conclusion: The ACE I/D polymorphism is closely associated with variance of its activity in the serum and isn't involved in the affinity between the active site and substrate HGG.

关键词(KeyWords): 激肽酶Ⅱ;基因表达;血清学
Kininase Ⅱ; gene expression; serology

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作者(Author): 黄海,陶建蜀,禄永汉
HUANG Hai, TAO Jian shu, LU Yong han (The Department of Clinical Biochemistry, the Medical Laboratory Sciences Faculty of Guiyang Medical College, Guiyang, China 550004)

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