非高渗培养蜡样芽胞杆菌L型研究A Study on L-form of Bacillus Cereus Cultured in Non-high Osmotic Media
王和;唐七义;徐英;
Wang He, et al Department of Microbiology
摘要(Abstract)：
用青霉素在L型培养基上诱导蜡样芽胞杆菌成为L型后直接种入无血清亦无渗透保护剂的非高渗透性1640液传代培养,观察其对渗透压和毛地黄皂苷的敏感性以及在非高渗透压环境中的形态、生长特性及返祖性。发现蜡样芽胞杆菌L型细胞表面粗糙,可通过0.22μm孔径超滤膜,对毛地黄皂苷不敏感和耐受0.8％～20％NaCl,一定浓度的葡萄糖可刺激多次传代的蜡样芽胞杆菌L型返祖。
In this paper L-form was induced from bacillus cereus by penicillin on the L-form medium, and then inoculated directly into non-high osmotic liquid medium, RPMI 1640 without serum or any osmotic protective agent, to subculture.Osmotic susceptibility, digitoninic susceptibility, characters of morphology, growth and reversibility of the L-form in non-high osmotic condions were observed.It was shown that the L-form cells pore rough surface and could pass through filter with 0.22μm pore.L-form of bacillus cereus is insensitive to digitonin and resistance to 0.8% to 20% NaCl.Reversion of the L-form subcultured in non-high osmotic medium without inducer could be stimulated by glucose.
关键词(KeyWords)：
蜡样芽胞杆菌;L型菌;渗透压
bacillus cereus;L-forms;osmotic pressure
基金项目(Foundation):
作者(Author):
王和;唐七义;徐英;
Wang He, et al Department of Microbiology
Email:
DOI: 10.19367/j.cnki.1000-2707.1993.02.008
参考文献(References)：
- 1 Domingue G J. Cell wall-defficient bacteria.London: Addison Wesley Publishing Co,1982． 101, 121
- 2 Madoff S. The bacterial L-form. New York: Marcel Dekker Inc, 1985． 5
- 3 Strickberger M W. Genetics. New York: Macmillan Publishing Co, 1985． 754
- 4 郭秉兰.细菌L型在鼠疫菌保存和传播中的意义.中华流行病学杂志,1989,10:292
- 5 王和.L型鸡蛋培养基的制备和细菌L型的诱导.贵阳医学院学报,1988,13:42
- 6 王和,等.用1640液培养细菌L型的研究.贵州医药,1989,13:324
- 7 王和,等.金黄色葡萄球菌L型菌落细胞渗透稳定性的测定.贵州医药,1989,13:199
- 8 Leon O. et al.Adaptation of an osmotically fragile L-form of strepto coccus pyogenes to physiological osmotic conditions and its obility to destroy human heart cells in tissue culture. Infect Immun, 1976, 13: 252
- 9 Takahashi T. Adaptation of a stable L-form of staphylococcus aureus to the medium with low osmolarity. Jaopan J Exp Med, 1980, 5:73
- 10 Partridge S M, et al. Isolation of cholesterol from the oily droplets found in association with the L1 organism separated from streptoba cillus moniliformis. J Pathol Bacterio, 1941, 52: 219
- 11 Nishiyama Y, et al. Morpholoical detection of filipin-sterol cmplexes in the cytoplasmic membrane of staphylococcal L-form. Microbiol Immunol, 1990, 34: 25
- 12 王宪楷,主编.天然药物化学.北京:人民卫生出版社,1988． 540
- 13 Razin S,et al. Methods in mycoplasmology. New York: Academic Press, 1983, 355
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