贵州医科大学学报

2006, (05) 393-395

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兔髁突细胞与可吸收明胶海绵复合培养的研究
A Study on the Biocompatibility Between Rabbit Mandibular Condylar Cells and Amnion

马敏先;贾莹;张忠平;王永;
MA Min-xian,JIA Ying,ZHANG Zhong-ping,WANG Yong(Department of Stomatology,The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,China)

摘要(Abstract):

目的:研究明胶海绵作为颞下颌关节组织工程支架材料应用的可行性。方法:采用胶原酶阶段消化法获取兔髁突软骨中的纤维软骨细胞,取经碱性成纤维细胞生长因子(bFGF)扩增的第3代细胞与已用多聚赖氨酸修饰的明胶海绵复合培养,进行噻唑蓝(MTT)比色及光镜、扫描电镜观察。结果:纤维软骨细胞于明胶海绵孔壁内牢固粘附,5d开始增殖,4W时有大量细胞生长于明胶海绵上。结论:明胶海绵表现出良好的亲水性、细胞吸附性和体外生物相容性,可作为组织工程细胞培养支架材料用。
Objective:To investigate the possibility of amnion as cell scaffold in tissue engineering of temporomandibular joint.Methods:Rabbit mandibular condylar fibrocartilage was digested by collagenase,and cells of the second generation proliferated by TGF-β1 were cultured onto amnion.Observation was made with MTT,light microscope,and electronic scanning microscopy.Results:Fibrocartilage cells attached to amnion closely and began to proliferate on the 3rd day.In 3 weeks after the transplantation,a lot of cells grew well on amnion.Conclusion:Amnion has good water adsorptivity and biocompatibility with fibrocartilage cells.It can be used as scaffold for tissue engineering of temporomandbular joint.

关键词(KeyWords): 颞下颌关节;下颌骨髁状突;软骨;细胞培养;明胶海绵,吸收性的;组织工程
temporomandibular joint;mandibular condyle;cartilage;cell culture;gelatin sponge,absorbable;tissue engineering

Abstract:

Keywords:

基金项目(Foundation): 贵州省优秀科技人才省长基金资助项目(S2005-2)

作者(Author): 马敏先;贾莹;张忠平;王永;
MA Min-xian,JIA Ying,ZHANG Zhong-ping,WANG Yong(Department of Stomatology,The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,China)

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DOI: 10.19367/j.cnki.1000-2707.2006.05.004

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