贵州医科大学学报

2008, No.131(02) 136-138

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原代肾小管上皮细胞的培养和鉴定
Culturing and Identification of Primary Generation of Rat Kidney Tubular Epithelial Cells

方开云;石明隽;肖瑛;桂华珍;郭兵;张国忠;
FANG Kaiyun,SHI Mingjun,XIAO Ying,GUI Huazhen,GUO Bing,ZHANG Guozhong

摘要(Abstract):

目的:建立大鼠肾小管上皮细胞原代培养及鉴定方法。方法:采用研磨、消化培养法,用免疫细胞化学和RT-PCR鉴定细胞。结果:培养的细胞CK18蛋白表达阳性,基本没有α-SMA和Vimentin的蛋白表达,E-cadherin mRNA强表达。结论:成功建立大鼠肾小管上皮细胞原代培养方法,为肾脏疾病的研究提供实验平台。
Objective:To establish a method for culturing and identifying the primary generation of rat renal tubular epithelial cells.Method:Rat renal tubular epithelial cells were collected with grinding and digesting methods,and the cell types were identified with immunocytochemistry and RT-PCR methods.Result:Strongly positive staining of CK18 and the expression of E-cadherin mRNA were observed in cells,while the expression of α-SMA and Vimentin proteins was hardly detected.Conclusion:The present method is efficient for the primary culturing of renal tubular epithelial cells.

关键词(KeyWords): 上皮细胞;肾小管;细胞培养;大鼠
epithelial cells;kidney tubules;cell culture;rats

Abstract:

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基金项目(Foundation): 贵州省高层次人才特助经费资助项目(2004-0517);; 教育部高等学校博士学科点专项科研基金(20060660001)

作者(Author): 方开云;石明隽;肖瑛;桂华珍;郭兵;张国忠;
FANG Kaiyun,SHI Mingjun,XIAO Ying,GUI Huazhen,GUO Bing,ZHANG Guozhong

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DOI: 10.19367/j.cnki.1000-2707.2008.02.008

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