贵州医科大学学报

2014, v.39;No.171(06) 809-812

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松油烯-4-醇对白念珠菌PLB1、SAP2基因表达的影响
The Effect of Terpinen-4-ol on PLB1 and SAP2 Gene Expression of Candida albicans

王梅竹;罗红梅;曹煜;瞿任;李小玲;赵亮;康颖倩;
WANG Meizhu;LUO Hongmei;CAO Yu;QU Ren;LI Xiaoling;ZHAO Liang;KANG Yingqian;Department of Microbiology,Guiyang Medical University;Department of Neonatology,Maternity and Child Care Hospital of Guiyang City;Department of Dermatology and Venereal Disease,Affiliated Hospital of Guiyang Medical University;

摘要(Abstract):

目的:观察中药抗菌成分松油烯-4-醇对白念珠菌磷脂酶B1(PLB1)和分泌型天冬氨酸蛋白酶2(SAP2)基因表达的影响。方法:将白念珠菌分为实验组和对照组,实验组用松油烯-4-醇作用12 h,对照组不做任何处理,提取两组白念珠菌总RNA,进行RT-PCR扩增,比较两组PLB1和SAP2的相对表达量。结果:用松油烯-4-醇作用后的白念珠菌PLB1和SAP2基因的相对表达量均高于没有用药的白念珠菌(P<0.05)。结论:松油烯-4-醇作用于白念珠菌可短时间内增加PLB1、SAP2 mRNA的表达。
Objective: To observe the effects of terpinen-4-ol,an antifungal ingredient of traditional chinese medicine,on phospholipase B1( PLB1) and secreted aspartic proteinase 2( SAP2) genes of Canidia albicans( C. albicans). Methods: C. albicans were divided into experiment group and control group,experiment group was treated with terpinen-4-ol for 12 h,control group didn't accept any treatment. Total RNA was extracted and amplified by RT-PCR. Relative expression levels of PLB1 and SAP2 mRNA were observed. Results: The relative expression levels of PLB1 and SAP2 gene of C. albicans exposed to terpinen-4-ol was higher than those of the control group( P < 0. 05). Conclusions:Terpinen-4-ol can increase the expression of PLB1 and SAP2 mRNA in the short time.

关键词(KeyWords): 念珠菌,白色;松油烯-4-醇;磷脂酶类;分泌型天冬氨酸蛋白酶2
Candida albicans;terpinen-4-ol;phospholipases;secreted aspartic proteinase 2

Abstract:

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基金项目(Foundation): 国家自然基金地方项目(31060006,31260029);; 贵州省社会发展科技攻关项目[黔科合SY字(2011)3017号];; 贵州省卫生厅科技项目(gzwkj2010-1-025);; 贵阳市科技局社会发展与民生计划[筑科合同(2011)10316号]

作者(Author): 王梅竹;罗红梅;曹煜;瞿任;李小玲;赵亮;康颖倩;
WANG Meizhu;LUO Hongmei;CAO Yu;QU Ren;LI Xiaoling;ZHAO Liang;KANG Yingqian;Department of Microbiology,Guiyang Medical University;Department of Neonatology,Maternity and Child Care Hospital of Guiyang City;Department of Dermatology and Venereal Disease,Affiliated Hospital of Guiyang Medical University;

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