贵州医科大学学报

1997, (04)

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贵州HCV感染株基因分型研究
The Study of HCV Genotype in Guizhou Area

丁静娟,杨京,田苗
Ding Jingjuan,et alDepartment of Infectious Diseases,the Affiliated Hospital of Guiyang Medical College

摘要(Abstract):

为了解本地区HCV感染株的基因型及现有分型方法的适用性,从贵州206例慢性肝炎和血液病患者中筛出35份抗-HCV阳性血清,用逆转录套式聚合酶链反应作HCVRNA检测。30份HCVRNA阳性血清用3种分型方法(核心区特异引物PCR,非结构5区特异探针核酸杂交,5′末端非编码区限制性长度多态性分析)进行HCV基因分型。结果3份血清为HCV2a感染,27份为HCV1b感染。对8份HCV1b、1份HCV2a血清的PCR扩增产物直接测序,核酸序列分型结果与原型别一致。表明HCV1b是贵州地区HCV感染株的主要基因型,HCV2a感染并不常见。以HCV基因组不同区域为基础的各方法分型结果间有很好的相符性。
Hepatitis C virus (HCV) has been classified at least 11 major genotype and 30 subtypes.The distribution of each genotype has been found to differ in different countries.Of 206 patients (100 with chronic hepatitis and 106 with hemopathy) from Guizhou province in southwestern China,only 30 (14%) were positive for HCV RNA as determined by RTNested PCR at the 5′ noncoding region.The HCV genotypes of these 30 patients were examined using polymerase chain reaction with typespecific primers at the core region and slotblot hybridization with typespecific cDNA probes at the NS5B region.Of 30 patients,27 cases had genotype 1b infection and other 3 cases had genotype 2a infection.The nucleotide sequence at the NS5B region of 8 patients with type 1b infection and one patients with 2a were analysed using the direct sequencing.The subtypes determined by sequence analysis were completely concordance with those decided by the two methods.These results indicated that the genotyping methods used in this study were valid and subtype 1b is the predominent HCV genotype in Guizhou area,subtype 2a is less common,other genotype was not been found.

关键词(KeyWords): 丙型肝炎病毒,基因型,聚合酶链反应,核酸杂交,核酸序列
hepatitis C virus genotype;blotting;primerset;polymerase chain reaction;direct sequence

Abstract:

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基金项目(Foundation): 贵州省科委基金

作者(Author): 丁静娟,杨京,田苗
Ding Jingjuan,et alDepartment of Infectious Diseases,the Affiliated Hospital of Guiyang Medical College

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DOI: 10.19367/j.cnki.1000-2707.1997.04.003

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