陈玉梅;杨文秀;刘运麟;方艺;孟青;
CHEN Yumei1,YANG Wenxiu1,LIU Yunlin2,FANG Yi1,MENG Qing1(1.Department of Pathology,Guiyang Medical College,Guiyang 550004,Guizhou,China;2. Division of Pathology,Qiannan District People's Hospital,Duyun 558000,Guizhou,China;)

• 1:贵阳医学院病理学教研室
• 2:贵州省黔南州人民医院病理科

摘要(Abstract)：

目的:检查CD5在淋巴结内弥漫大B细胞淋巴瘤(DLBCL)中的表达情况,了解CD5表达与肿瘤细胞中CD10、bcl-6、MUM1表达的关系,以及上述分子标记对肿瘤的临床病理特征和预后的影响。方法:HE染色观察淋巴结内DLBCL的组织学特征,免疫组织化学染色检测CD5、CD10、bcl-6、MUM1和Ki-67表达。结果:39例DLBCL中25例表达CD5,DLBCL的CD5阳性和阴性组的CD10,bcl-6,MUM1、Ki-67的表达、年龄、性别、体能指数及生存状况无显著性差异,但两组的临床分期有显著性差异。CD10(-)bcl-6(-)MUM1阳性组与阴性组之间生存状况无显著性差异,CD10和(或)bcl-6表达组与MUM1表达组及Ki67表达>20%与≤20%病例之间生存状况无明显差异。结论:DLBCL中CD5表达与CD10、bcl-6、MUM1的表达无明显相关,但与肿瘤的临床分期有关,CD5阴性病例的分期较低;CD5表达对DLBCL的其它临床特征和生存状况无明显影响,CD10、bcl-6、MUM1表达对DLBCL的生存状况有一定影响。
Objective:To detect the expression of CD5 in nodal diffuse large B cell lymphoma (DLBCL) and understand the correlation between CD5 expression and the expression of CD10,bcl-6,and MUM1 in DLBCL as well as its effect on the clinicopathological features and prognosis of DLBCL. Methods: Pathological specimens of 39 cases of DLBCL were collected and their histopatholigical features were observed with HE stained sections. Some immunophenotypes of DLBCL ( CD5,CD10,bcl-6,and MUM1,etc.) and Ki-67 expression were detected by using immunohistochemistry staining. Result: Expression of CD5 was detected in 25 DLBCL cases out of 39. There was no statistical difference of expression of CD10,bcl-6,MUM1,or Ki-67 between CD5-postive (CD5+) and -negative (CD5-) groups,neither the survival status or clinicopathological features such as age,sex and so on were statistically different between the 2 groups. While there were indeed statistical differences of staging between the two groups. The differences of survival status between CD10-,bcl-6-,and MUM1-postive and -negative groups were not significant. There was no obvious difference of survival between CD10/ bcl-6-postive and MUM1-postive groups,or between Ki-67≥20% and <20% groups. Conclusions: The expression of CD5 is not related to the expression of CD10,bcl-6,MUM1 in DLBCL,but is related to the staging of DLBCL. The stages are lower in CD5-negative cases than those in CD5-postive cases. CD5 expression is not associated with other clinical features or survival status of DLBCL. The expressions of CD10,bcl-6,MUM1 affect the prognosis of DLBCL.

关键词(KeyWords)： 淋巴瘤,B细胞;CD5;CD10;bcl-6;MUM1
lymphoma;B-cell;CD5;CD10;bcl-6;MUM1

Abstract:

Keywords:

基金项目(Foundation): 贵州省省长基金资助项目(S2004-18);; 贵阳医学院博士启动基金(C2005-1);; 贵州省卫生厅科技项目(G2005-2);; 贵阳医学院2009年研究生教育创新基地专项经费(S200906)

作者(Author): 陈玉梅;杨文秀;刘运麟;方艺;孟青;
CHEN Yumei1,YANG Wenxiu1,LIU Yunlin2,FANG Yi1,MENG Qing1(1.Department of Pathology,Guiyang Medical College,Guiyang 550004,Guizhou,China;2. Division of Pathology,Qiannan District People's Hospital,Duyun 558000,Guizhou,China;)

Email:

DOI: 10.19367/j.cnki.1000-2707.2009.05.004

参考文献(References)：

• [1]Gaiter K,Warnke RA.Difuse large B-cell lymphoma[M]//Jaffe ES,Stein H,Vardiman JW,eds,World heahh organization classification of tumours:Pathologyand Genetics of Tumours of Haematopoietic and Lymphoid Tissues.Lyon,France:IARC Press,2001:171-174.
• [2]Wright G,Tan B,Rosenwald A,et al.A gene expres-sion-based method to diagnose clinically distinct subgroup s of diffuse large B-cell lymphoma[J].Proc NatlAcad SciUSA,2003(17):9991-9996.
• [3]Katzenberger T,LohrA,Schwarz S,et al.Genetic analy-sis of de novo CD5+diffuse large B-cell lymphomas sug-gests an origin from a somatically mutated CD5+progen-itor B cell[J].Blood,2003(2):699-702.
• [4]YamaguchiM,SetoM,OkamotoM,et al.Denovo CD5diffuse large B-cell lymphoma:aclini-copathologicstudy of109pa-tients[J].Blood,2002(99):815-821.
• [5]Alizadeh AA,EisenMB,Davis RE,et al.Distinct types of diffuse large B-cell lymphoma identified by gene expres-sion p rofiling[J].Nature,2000(6769):503-511.
• [6]Rosenwald A,Wright G,ChanWC,et al.The use of mo-lecular profiling to predict survival after chemotherapy for diffuse large B-cell lymphoma[J].N Engl J Med,2002(25):1937-1947.
• [7]Natkunam Y,Warnke R,Montgomery K,et al.Analysis of MUM1IRF4protein expression using tissue microarrays and immunohistochemistry[J].Mod Pathol,2001(7):686-694.

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