贵州医科大学学报

2017, v.42;No.206(11) 1274-1278

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几株真菌对白酒丢糟纤维素水解能力的研究
The Study on Hydrolysis Ability of Several Fungi to Cellulose in Waste Distiller's Grains

明春艳;王颜颜;曹煜;邱婷婷;夏茂宁;牛雪可;邱文;万会燕;康颖倩;
MING Chunyan;Wang Yanyan;CAO Yu;QIU Tingting;XIA Maoning;NIU Xueke;QIU Wen;WAN Huiyan;KANG Yingqian;Key Laboratory of Pathogenic Biology of Guizhou High Education Institution & Key Laboratory of Environmental Pollution Monitoring and Disease Control of Ministry of Education;Department of Microbiology,Guizhou Medical University;Department of Dermatology and Venereal Disease,Affiliated Hospital of Guizhou Medical University;

摘要(Abstract):

目的:筛选对丢糟中纤维素水解能力强的真菌菌株。方法:选择7株具有水解纤维素酶的真菌,以羧甲基纤维素钠(CMCNa)为唯一碳源的培养基进行初筛、以丢糟为唯一营养物质的酸性固体或液体培养基培养,观察培养24 h时培养基上水解圈及真菌直径,测定液体培养基中丢糟的减重率及纤维素的水解率,筛选高效水解丢糟中纤维素的真菌。结果:从7株真菌中筛选出G-7 Trichoderma reese,G-1 Phanerochaete chrysosporium,G-2 Trichoderma longibrachiatum,G-3 Trichoderma longibrachiatum 4株高效水解丢糟中纤维素的真菌,在CMCNa-刚果红培养基上培养6 d的水解圈直径D/菌饼直径d(D/d))为6.0∶7.5、1.7∶2.7、5.2∶7.5、5.0∶7.5;在丢糟酸性固体培养基上G-7、G-1、G-2、G-3生长良好,对丢糟的降解能力依次是65.73%、61.90%、61.22%、59.62%,对丢糟中纤维素的水解大小依次是57.62%、54.22%、49.41%、44.21%。结论:G-1、G-2、G-3、G-7为高效水解菌株;利用CMCNa-刚果红培养基判断菌株对纤维素的水解能力具有一定的参考价值;利用丝状真菌处理丢糟,有利于丢糟的转化利用。
Objective: To study on screening of fungal strains in the lost cellulose hydrolysis ability.Method: 7 strains of fungi with cellulase hydrolysis were bought from Westerdijk Fungal Biodiversity institute of CBS. The medium with sodium carboxymethyl cellulose( CMCNa) as the sole carbon source was screened,and the acidic solid and liquid medium with distiller's grains as the sole nutrient were screened. At the same time,the weight loss rate and hydrolysis rate of cellulose in liquid medium were determined. Results: G-7 Trichoderma reese,G-1 Phanerochaete chrysosporium,G-2 Trichoderma longibrachiatum and G-3 Trichoderma longibrachiatum were screened from 7 strains of fungi to hydrolyze cellulose fungi in the lees. After Cultivating for 6 days,the hydrolytic circles of tested strains were respectively 6. 0: 7. 5,1. 7: 2. 7,5. 2: 7. 5 and 5. 0: 7. 5 on sodium carboxymethyl cellulose-Congo red medium. G-7,G-1,G-2 and G-3 grew well on the acid solid medium. The degradation ability of grains were 65. 73%,61. 90%,61. 22% and 59. 62%,and the cellulose hydrolysis rate of distiller's grains were 57. 62%,54. 22%,49. 41% and 44. 21%. Conclusion: In this study,we not only determine some efficient fungal strain on cellulose hydrolysis of waste distiller 's grains,but also build the comprehensive method on fungal selection for degradation of waste distiller's grains.

关键词(KeyWords): 真菌;丢糟;纤维素酶;水解;培养基;酒糟
fungi;waste distiller's grains;cellulose;hydrolysis;medium;lees

Abstract:

Keywords:

基金项目(Foundation): 贵阳国家高新技术产业开发区管理委员会高层次人才创新创业项目(2016);; 贵州省国际科技合作项目[黔科合外G字(2014)7006];; 贵阳市人民政府-贵州医科大学科学技术联合基金“贵阳市科技局创新团队”项目(GY2015-18);; 贵阳市科学技术局科技开发计划项目[筑科合同(20151001)社18号];; 贵阳市科技计划项目[筑科合同(20161001)005号]

作者(Author): 明春艳;王颜颜;曹煜;邱婷婷;夏茂宁;牛雪可;邱文;万会燕;康颖倩;
MING Chunyan;Wang Yanyan;CAO Yu;QIU Tingting;XIA Maoning;NIU Xueke;QIU Wen;WAN Huiyan;KANG Yingqian;Key Laboratory of Pathogenic Biology of Guizhou High Education Institution & Key Laboratory of Environmental Pollution Monitoring and Disease Control of Ministry of Education;Department of Microbiology,Guizhou Medical University;Department of Dermatology and Venereal Disease,Affiliated Hospital of Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2017.11.008

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