贵州医科大学学报

2012, v.37;No.158(05) 473-475

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不同日龄白纹伊蚊雌蚊唾液腺总蛋白谱分析
Analysis on Salivary Gland Protein Profile of Female Aedes albopictus at Different Age

刘鉴;孙宇;程金芝;陈璐;吴家红;
LIU Jian,SUN Yu,CHENG Jinzhi,CHEN Lu,WU Jiahong(Department of Parasitology & Basic Laboratory of Modern Pathogenic Biology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

摘要(Abstract):

目的:探讨不同日龄白纹伊蚊雌蚊唾液腺蛋白含量及总蛋白谱差异。方法:分别采用Pierce蛋白定量试剂盒和SDS-PAGE电泳方法,分析白纹伊蚊雌蚊各日龄唾液腺蛋白质定量及蛋白谱。结果:0日龄每对唾液腺蛋白质质量为(0.677±0.30)μg,3日龄为(1.454±0.46)μg,5日龄为(1.95±0.38)μg,7日龄为(1.733±0.46)μg,10日龄为(1.205±0.43)μg;SDS-PAGE检测到0日龄唾液腺总蛋白有12条主带,分别为15 kDa、18 kDa、30 kDa、32 kDa、34 kDa、40 kDa、45 kDa、48 kDa、50 kDa、62 kDa、68 kDa、>116 kDa 1条,还有其它比较弱的次带;3日龄以后的唾液腺总蛋白有12条带,但少了40 kDa条带,多了28 kDa条带。结论:不同日龄白纹伊蚊雌蚊唾液腺蛋白谱有一定差异。
Objective: To explore the difference of the salivary gland protein content and profile among female Aedes albopictus at different age.Methods: The contents of salivary gland proteins were detected with Pierce protein assay kit,and protein profile was determined with SDS-PAGE electrophoresis.Results: The amount of salivary gland proteins of 0 day,3 days,5 days,7days and 10 days old female Aedes albopictus was 0.677±0.30μg,1.454±0.46μg,1.95±0.38μg,1.733±0.46μg and 1.205±0.43μg respectively.Twelve major bands of 15,18,30,32,34,40,45,48,50,62,68kDa and a band > 116 kDa of salivary gland proteins of 0 day old female mosquitoes were showed by SDS-PAGE electrophoresis.There were also 12 major bands for older female mosquitoes but with 28kDa instead of 40kDa.Conclusions: There are certain differences in the salivary gland profile among female Aedes albopictus at different age.

关键词(KeyWords): 伊蚊属;涎腺;蛋白质组
aedes;salivary glands;proteome

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(3060515,81060138);; 贵州省省长基金【黔省专合字(2007)48号】

作者(Author): 刘鉴;孙宇;程金芝;陈璐;吴家红;
LIU Jian,SUN Yu,CHENG Jinzhi,CHEN Lu,WU Jiahong(Department of Parasitology & Basic Laboratory of Modern Pathogenic Biology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

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DOI: 10.19367/j.cnki.1000-2707.2012.05.005

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