管志敏;柏晓辉;屈璐;赵大尉;刘颖;丁优;潘艳伶;
GUAN Zhimin;BAI Xiaohui;QU Lu;ZHAO Dawei;LIU Ying;DING You;PAN Yanling;Department of Traditional Chinese Medicine,The Affiliated Hospital of Guizhou Medical University;

• 1:贵州医科大学
• 2:贵州医科大学附院中医科

摘要(Abstract)：

目的:探讨穴位埋线、糖通饮防治早期糖尿病肾病(DN)的作用及机理。方法:60只SD大鼠随机分为正常对照组(12只)和造模组(48只),造模组大鼠采用腹腔注射STZ方法制作早期ND模型;造模成功后,随机分为模型组12只和治疗组(中药组、穴埋组、中药+穴埋组即药埋组,每组12只),根据分组进行相应的治疗4周,对照组和模型组灌胃生理盐水4周;疗程结束后72 h,剪尾取血测空腹血糖(FBG),检测血清和转化生长因子-β1(TGF-β1)及胰岛素样生长因子-1(IGF-1)水平,同时取大鼠肾组织计算肾脏肥大指数(KHI)。结果:治疗组(中药组、穴埋组、药埋组)大鼠较模型组大鼠FBG、TGF-β1、IGF-1血清水平含量显著降低(P<0.01),药埋组尤为明显;治疗组大鼠较模型组大鼠KHI明显降低(P<0.01)。结论:穴位埋线及糖通饮能有效降低早期DN大鼠TGF-β1、IGF-1血清水平含量,是防治早期DN的有效方法。
Objective: To preliminarily study effects and the possible mechanism of catgut embedding therapy and Tangtong drink on early DN rats. Methods: Sixty SD rats were randomly divided into the normal group( 12 rats) and the model group. The model group received intraperitoneal injection of STZ combined with high energy diet to establish early DN rats model. After model building,these model rats were divided into non-treatment group( 12 rats),and treatment group including Chinese medicine group,embedding group and the embedding plus Chinese medicine group,12 rats in each group. The treatment lasted 4 weeks and normal group and model group received intragastric administration of normal saline for 4 weeks. 72 hours after treatment,all rats were killed and the tail blood was collected to detect FBG level and serum TGF-β1,IGF-1level. Meanwhile,the rats' kidney tissue was taken to calculate the KHI. Results: Compared with non-treatment group,the FBG level and serum TGF-β1,IGF-1 level of the treatment group( Chinese medicine group,embedding group and the embedding plus Chinese medicine group) decreased significantly( P < 0. 01),of which,the embedding plus Chinese medicine group showed the most significant decrease. The kidney hypertrophy index( KHI) in rats of treatment group were significantly reduced compared with the non-treatment group( P < 0. 01). Conclusions: Catgut embedding therapy and Tangtong drink can reduce early DN rats'FBG level,TGF-β1level,IGF-1 level,and KHI. It is an effective method for the prevention and treatment of early DN.

关键词(KeyWords)： 穴位;埋线疗法;糖通饮;糖尿病肾病;转化生长因子β1;胰岛素样生长因子1
acupoints;embedding therapy;Tangtong drink;diabetic nephropathy;transforming growth factor beta 1;insulin like growth factor-1

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 管志敏;柏晓辉;屈璐;赵大尉;刘颖;丁优;潘艳伶;
GUAN Zhimin;BAI Xiaohui;QU Lu;ZHAO Dawei;LIU Ying;DING You;PAN Yanling;Department of Traditional Chinese Medicine,The Affiliated Hospital of Guizhou Medical University;

Email:

DOI: 10.19367/j.cnki.1000-2707.2015.12.017

参考文献(References)：

• [1]徐丽,张红.芪母益肾煎抗实验性糖尿病肾病作用及其可能机制[J].中国临床药学杂志,2007(4):218-220.
• [2]Flyvbjerg A.Putativepathophysiologicalrole of growth factors and cytokines in experimental diabetic kidney disease.Diabetologia,2000(43):1205.
• [3]Kim NH,Jung HH,Cha DR,et al.Expression of vascular endo helial growth factor in response to high Glucose in rat mesangial cells[J].Endocrinology,2009(165):617.
• [4]Nerlich A,Schliecher E.Immunohis To Chemical Localization of extra cellular matrix components in human diabetic glomerular lesions[J].Am JPathol,1991(139):889.
• [5]于倩,张沫,刘德敏.TGF-β1、CTGF基因的过表达与早期糖尿病肾病关系的研究[J].天津医药,2012(3):262-265.
• [6]罗小光,夏佩,胡选霞.穴位穴埋和中药预防和治疗雌性大鼠卵巢储备功能下降及卵巢早衰的实验研究[J].中华中医药杂志,2014(2):423-426.
• [7]刘丽燕,黄吉峰,王茗龚,等.肾白宁对早期糖尿病肾病大鼠模型蛋白非酶糖化终末产物(AGEs)的影响[J].中医药信息,2011(1):63-64.
• [8]Schrijvers BF,Flyvbjerg A,Tilton RG,et al.Aneutralizing VEGF antibody prevents glomerular hypertrophy in a model of obese type 2 diabetes,the Zucker diabetic fatly rat[J].Nephrol Dial Transplant,2006(2):324-329.
• [9]赵谭,孙树印,吴玉梅.胰岛素生长因子-1在2型糖尿病肾病进展中的作用[J].山东医药,2012(37):78-80.
• [10]Boor P Sebekova K,Oatendorf T,et al.Treatment targets in renol fibrosis[J].Nephrol Dial Transplant,2007(22):3391-3407.
• [11]林兰.现代中医糖尿病学[M].北京:人民卫生出版社,2008:542.
• [12]陈永斌,陈仁年.穴位埋线为主干预2型糖尿病早期肾病[J].中国针灸,2012(5):390-393.
• [13]张存志,张瑞君,邸力强.中药注射液加用经穴穴埋对早期糖尿病肾病患者微血管病变的影响[J].中医杂志,2007(1):50-52.

文章评论(Comment)：