宋洋;杨艳;任芳;
SONG Yang;YANG Yan;REN Fang;Shengjing Hospital Affiliated to China Medical University;

• 1:中国医科大学附属盛京医院

摘要(Abstract)：

目的:探讨lncRNA SNHG1在卵巢癌组织中的表达及其对卵巢癌细胞增殖的影响。方法:采用Real-time PCR检测22对卵巢癌组织、癌旁组织以及5株卵巢癌细胞株中lncRNA SNHG1的表达,选择两株lncRNA SNHG1表达最高的细胞瞬时转染NC siRNA(NC siRNA组)或者lncRNA SNHG1 siRNA(lncRNA SNHG1 siRNA组);实验设置对照组,采用CCK-8检测各组细胞的增殖情况,双荧光素酶检测lncRNA SNHG1对miR-199a-3p的调控作用。结果:Real-time PCR结果显示,lncRNA SNHG1在卵巢癌组织中的表达水平显著高于癌旁组织,OV-90、CaOV3细胞株lncRNA SNHG1的表达水平明显高于其他卵巢癌细胞株,差异有统计学意义(P<0.05);与NC siRNA组比较,lncRNA SNHG1 siRNA组细胞lncRNA SNHG1的表达水平显著降低,差异有统计学意义(P<0.05);CCK-8结果显示在OV-90和CaOV3细胞株中下调lncRNA SNHG1均显著抑制了细胞的增殖(P<0.05);双荧光素酶结果显示野生型lncRNA SNHG1和miR-199a-3p mimic共转染细胞显著降低了双荧光素酶的活性,差异有统计学意义(P<0.05)。结论:lncRNA SNHG1与卵巢癌的进展密切相关,lncRNA SNHG1有望成为治疗和预防卵巢癌的潜在靶点。
Objective: To evaluate the expression of lncRNA SNHG1 in ovarian cancer tissue and investigate its effect on the proliferation of ovarian cancer cells. Methods: The expression of lncRNA SNHG1 in 22 pairs of ovarian cancer tissue and the adjacent tissue were determined by real-time PCR; the expression of lncRNA SNHG1 in 5 ovarian cancer cell lines was also detected. Two cell strains with the highest expression of lncRNA SNHG1 were selected for transient transfection of NC siRNA or lncRNA SNHG1 siRNA. Cell proliferation activities were examined by CCK-8 assay. Dual-Luciferase reporter assay was also used to exam whether lncRNA SNHG1 has the microRNA response elements of miR-199a-3p. The control group, NC siRNA group and lncRNA SNHG1 siRNA group were set up, and proliferation of the cells in each group was detected by CCK-8. Regulation of lncRNA SNHG1 on miR-199 a-3 p was detected by Dual-Luciferase. Result: Results of Real-time PCR showed that the expression level of lncRNA SNHG1 in ovarian cancer tissue was significantly higher than that of the cancer-adjacent tissue(P<0.05). The expression levels of lncRNA SNHG1 in OV-90 and CaOV3 cell strains were significantly higher than those in other ovarian cancer cell strains(P<0.05). Compared with the NC siRNA group, the expression level of lncRNA SNHG1 in lncRNA SNHG1 siRNA group significant decreased(P<0.05).The results of CCK-8 showed that down-regulation of lncRNA SNHG1 in both OV-90 and CaOV3 cell strains significantly inhibited cell proliferation(P<0.05). Dual-Luciferase reporter assay showed that co-transfection with wild type lncRNA SNHG1 and miR-199a-3p mimic significantly reduced the activity of luciferase, and differences were statistically significant(P<0.05). Conclusions: lncRNA SNHG1 is closely related to the development of ovarian cancer, lncRNA SNHG1 may be a novel target in the prevention and treatment of ovarian cancer.

关键词(KeyWords)： 卵巢肿瘤;癌;lncRNA SNHG1;增殖;RNA干扰;转染
ovarian cancer;carcinoma;lncRNA SNHG1;proliferation;RNA interference;transfection

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年科学基金项目(81501235)

作者(Author): 宋洋;杨艳;任芳;
SONG Yang;YANG Yan;REN Fang;Shengjing Hospital Affiliated to China Medical University;

Email:

DOI: 10.19367/j.cnki.1000-2707.2019.05.008

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