贵州医科大学学报

2016, v.41;No.191(08) 869-873

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凋亡诱导因子在阿尔茨海默病患者大脑中的表达及意义
Expression and Significance of Apoptosis Inducing Factor in the Brain of Alzheimer's Disease

杨梅;任真奎;禹文峰;官志忠;
YANG Mei;REN Zhenkui;YU Wenfeng;GUAN Zhizhong;Key Lab of Medical Molecular Biology,Guizhou Medical University;

摘要(Abstract):

目的:了解凋亡诱导因子(AIF)在阿尔茨海默病患者(AD)脑组织中的表达及亚细胞分布。方法:7例AD患者(AD组)和7例正常老年人(对照组)大脑组织标本,采用免疫组织化学染色法,记录并比较被检脑组织海马CA1、CA2、CA3、DG、内嗅皮质浅层(ECⅠ~Ⅲ)和深层(ECⅣ~Ⅵ)以及颞叶浅层(TCⅠ~Ⅲ)和深层(TCⅣ~Ⅵ)区AIF蛋白阳性表达细胞数,计算AIF总评分和AIF核转移分比。结果:与对照组比较,AD组AIF蛋白在海马CA1、DG以及ECⅠ~Ⅲ区表达显著升高(P<0.05),在海马CA2、CA3、ECⅣ~Ⅵ及TCⅣ~Ⅵ区的表达无明显变化(P>0.05);AD组AIF细胞核转移分比在海马的CA1、CA2、CA3、DG区,内嗅皮质和颞叶皮质均显著高于对照组(P<0.05)。结论:AIF的细胞核转移可能是AD患者大脑中发生神经细胞凋亡的重要病理因素之一。
Objective: To explore the expression and subcellular distribution of apoptosis inducing factor( AIF) in the brain of patients with Alzheimer's disease( AD). Methods: Brain tissue were collected from 7 cases of AD patients( AD group) and 7 cases of healthy old people as controls( control group). Immunohistochemical staining was adopted to record the cell number of AIF protein positive expression in hippocampal CA1,Ca2,CA3 and DG area,in entorhinal cortex shallow layer( EC Ⅰ ~Ⅲ) and deep layer( EC Ⅳ ~ Ⅵ) and temporal lobe shallow layer( TC Ⅰ ~ Ⅲ) and deep layer( TCⅣ ~ Ⅵ). The AIF total score and AIF nuclear transfer ratio were calculated and compared between the two groups. Results: Compared with control group,AIF expression in hippocampal CA1,DG and EC Ⅰ ~ Ⅲ area increased significantly in AD group( P < 0. 05) while AIF expression showed no significant change in CA2,CA3,EC Ⅳ ~ Ⅵ and TC Ⅳ ~ Ⅵ area in AD group( P > 0. 05). Compared with control group,AIF nuclear transfer ratios in hippocampal CA1,Ca2,CA3,DG area,entorhinal cortex layer and temporal lobe layer were significantly higher in AD group( P < 0. 05). Conclusion:The nuclear transfer of AIF may be one of the important pathological factors of neuronal apoptosis in the brain of AD patients.

关键词(KeyWords): 阿尔茨海默病;脑;海马;细胞凋亡;凋亡诱导因子
Alzheimer's disease;brain;hippocampus;apoptosis;apoptosis-inducing factor

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81360199);; 教育部科学技术研究项目(213032A);; 贵州省国际科技合作项目[黔科合外G字(2013)7026号]

作者(Author): 杨梅;任真奎;禹文峰;官志忠;
YANG Mei;REN Zhenkui;YU Wenfeng;GUAN Zhizhong;Key Lab of Medical Molecular Biology,Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2016.08.001

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