贵州医科大学学报

1994, (02)

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

恶性疟疾重组质粒pPF_(14)DNA探针的制备
The Preparation of Recombinant Clone pPF_(14)DNA Probes

王季石,陆惠民,潘卫庆,缪为民,李卓江
Wang Jishi et al (Department of Internal Medicine, Guiyang Medical College)

摘要(Abstract):

采用基因克隆技术,将恶性疟原虫(Plasmodiumfalciparum)pPF14质粒转化大肠杆菌HB101,经扩增后碱裂解法提取质粒DNA,核酸内切酶酶切图谱分析鉴定后,低熔点胶法回收而获得高纯度和高回收率的插入片段(pPF14DNA),经32P标记后具较高敏感性与特异性,该探针制备方法具简便、稳定、省时的优点。本文对各制备程序进行了探讨。
This article described the praparation of pPF14 DNA probes. These procedures involved transformation of Escherichia coli strain HB 101, Amplification, plasmid was isolated by alkaline method and was dounbly digested with the restriction enzymes Hind Ⅲ and SaⅠ, pPF14 DNA fragment afterwards was analyzed by agarose gel electrophoresis and low melting gel methed recovered. The results showed that high purity and efficient pPF14 DNA fragment was obtained when using radiolabelled. The probe had a high sensitivity and speciality. The method was much easer,faster, stable and economical than other means, mean while, we discussed these procedures from defferent angles.

关键词(KeyWords): 恶性疟原虫,pPF_(14)DNA探针,核酸内切酶类,分子克隆
plasmodium falciparum; pPF_14 DNA probe; endonucleases; molecular cloning

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 王季石,陆惠民,潘卫庆,缪为民,李卓江
Wang Jishi et al (Department of Internal Medicine, Guiyang Medical College)

Email:

DOI: 10.19367/j.cnki.1000-2707.1994.02.003

文章评论(Comment):

序号(No.) 时间(Time) 反馈人(User) 邮箱(Email) 标题(Title) 内容(Content)
反馈人(User) 邮箱地址(Email)
反馈标题(Title)
反馈内容(Content)
扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享