贵州医科大学学报

2008, (04) 344-346

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RhD阳性血影细胞的制备及应用
Preparation and Application of Ghost Cells from RhD Positive Blood

石明隽;肖瑛;桂华珍;张国忠;
SHI Mingjun,XIAO Ying,GUI Huazhen,ZHANG Guozhong (Department of Pathophysiology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

摘要(Abstract):

目的:探讨用不同渗透浓度磷酸盐液(PB)制备的RhD阳性血影细胞的抗原性及其在抗-D抗体分离中的作用。方法:用不同渗透浓度PB作为裂解液,将遗传表现型为CCDee的RhD阳性O型红细胞制备成血影细胞,观察其抗原性;以血影细胞为固相抗原,用亲和层析法从抗D含量为0.814 mg/L的健康人血浆中分离抗-D抗体,检测相关指标。结果:pH7.4、30 mmol/L PB制备的血影细胞形态完整,保持较强的抗原性;成功获得抗-D抗体,回收率达到84.65%,IgG单体加二聚体含量为99.3%,不含抗A、抗B血型抗体以及IgA、IgM和血红蛋白。结论:用pH7.4、30 mmol/L PB制备的血影细胞可作为固相抗原,并能从低效价血浆中分离纯化抗-D抗体。
Objective: To investigate the antigenicity of RhD ghost cells prepared with different concentrations of phosphate solution(PB) and its application in the separation of anti-D antibody from plasma with low antibody titer.Methods:Red blood cells of O group with positive RhD(genotype CCDee) were employed to prepare ghost cells using PB with concentrations of 40,30 and 20 mmol/L respectively.Antigenicity of these cells was examined.Anti-D antibody was separated from the plasma containing 0.814 mg/L of anti-D by using affinity chromatography(AC) with the ghost cells as solid antigen.Results: 1.The ghost cells prepared with PB(pH 7.4,30 mmol/L) kept complete cell morphology and strong antigenicity;2.The anti-D antibody was separated successfully using AC method from plasma.The recovery rate of anti-D was 84.65%,and contents of monomer and dimmer reached 99.3% of total IgG.No IgA,IgM or hemoglobin was found in the preparation.Conclusion: The ghost cells prepared by PB(pH 7.4,30 mmol/L) can be used as solid antigen to separate and purify anti-D antibody from plasma with low anti-D titter.

关键词(KeyWords): RhD抗原;抗-D抗体;血影细胞;血浆
RhD antigen,anti-D antibody;ghost cells;plasma

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基金项目(Foundation): 国家科技部攻关项目资助(2002BA901A19)

作者(Author): 石明隽;肖瑛;桂华珍;张国忠;
SHI Mingjun,XIAO Ying,GUI Huazhen,ZHANG Guozhong (Department of Pathophysiology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

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