贵州医科大学学报

2006, (04) 327-329

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兔髁突软骨细胞与羊膜间生物相容性研究
A Study on the Biocompatibility Between Rabbit Mandibular Condylar Cells and Amnion

马敏先;王永;张忠平;贾莹;
MA Min-xian,WANG Yong,ZHANG Zhong-ping,JIA Ying(Department of Stomatology,The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,China)

摘要(Abstract):

目的:研究羊膜作为颞下颌关节组织工程支架材料应用的的可行性。方法:采用Ⅱ型胶原酶消化解离兔髁突软骨中的纤维软骨细胞,取经TGF-β1扩增的第二代细胞与羊膜复合培养,进行MTT、光镜、扫描电镜观察。结果:髁突细胞于羊膜上牢固黏附,第3天开始增殖,3周时已有大量细胞生长于羊膜上。结论:羊膜表现出良好的吸水性、细胞吸附性和体外生物相容性,髁突细胞可在其上黏附、增殖,羊膜适于作为髁突组织工程支架材料。
Objective: To investigate the possibility of amnion as cell scaffold in tissue engineering of temporomandibular joint.Methods: Rabbit mandibular condylar fibrocartilage was digested by collagenase,and cells of the second generation proliferated by TGF-β1 were cultured onto amnion.Observation was made with MTT, light microscope,and electronic scanning microscopy.Results: Fibrocartilage cells attached to amnion closely and began to proliferate on the 3rd day.In 3 weeks after the transplantation,a lot of cells grew well on amnion.Conclusion: Amnion has good water adsorptivity and biocompatibility with fibrocartilage cells.It can be used as scaffold for tissue engineering of temporomandbular joint.

关键词(KeyWords): 颞下颌关节;下颌骨髁状突;软骨细胞;羊膜;组织工程
temporomandibular joint;mandibular condylar;chondrocytes;amnion;tissue engineering

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作者(Author): 马敏先;王永;张忠平;贾莹;
MA Min-xian,WANG Yong,ZHANG Zhong-ping,JIA Ying(Department of Stomatology,The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,China)

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DOI: 10.19367/j.cnki.1000-2707.2006.04.011

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