贵州医科大学学报

2017, v.42;No.206(11) 1287-1291+1296

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移植前胚胎培养液IL-8在评价胚胎质量和妊娠结局中的价值
Value of Interleukin-8 in Embryo Culture Fluid on Evaluating Embryo Quality and Pregnancy Outcome before Implantation

黄官友;龙爱专;赵淑云;周桦;杨朝;田维婷;
HUANG Guanyou;LONG Aizhuan;ZHAO Shuyun;ZHOU Hua;YANG Chao;TIAN Weiting;Reproductive Medicine Center of Affiliated Hospital of Guizhou Medical University;

摘要(Abstract):

目的:探讨移植前人胚胎培养液中白细胞介素-8(IL-8)在评价胚胎质量和妊娠结局中的价值。方法:选取330例行体外受精-胚胎移植(IVF/ICSI)前胚胎培养液标本作为研究组,再将研究组分为IL-8阳性组(IL-8>0.19 ng/L)和IL-8阴性组(IL-8≤0.19 ng/L);另选9例没有放置胚胎的培养液作为阴性对照;采用液相芯片高通量蛋白质分析技术(LHTPA)测定培养液中IL-8含量,比较阴性对照组、IL-8阳性组和IL-8阴性组移植前人胚胎培养液中IL-8水平,比较IL-8阳性组和IL-8阴性组孕妇的年龄、不孕时间、病因(原发不孕、继发不孕及输卵管因素)、胚胎移植周期数、获卵数、移植胚胎数及妊娠期失访率,比较普通胚胎和优质胚胎移植组被检者移植前胚胎培养液中IL-8阳性和阴性构成及妊娠率,比较IL-8阳性组和IL-8阴性组妊娠率、临床妊娠率、种植率、生化妊娠率、流产率、活产率及活婴出生例数与接受胚胎移植患者总例数比值(NLBPP)。结果:研究组IL-8的阳性率为32.42%,IL-8阳性组中IL-8水平高于阴性对照组和IL-8阴性组(P<0.05);IL-8阳性组和IL-8阴性组被检者的年龄、不孕时间、病因(原发不孕和继发不孕)、胚胎移植周期数、注射HCG日的子宫内膜厚度、获卵数、移植胚胎数和妊娠期失访率比较,差异均无统计学意义(P>0.05);IL-8阳性组和IL-8阴性组移植优质或普通胚胎构成比比较,差异无统计学意义(P=0.76);IL-8阳性组移植优质胚胎的妊娠率高于IL-8阴性组(P=0.02),而移植普通胚胎的妊娠率,差异无统计学意义(P=0.82),IL-8阳性组患者妊娠率、胚胎种植率显著高于IL-8阴性组(P<0.05);其余指标比较差异无统计学意义(P≥0.05)。结论:移植前人胚胎培养液中IL-8可作为评价胚胎发育潜能的指标。
Objective: To explore the value of interleukin-8( IL-8) in embryo culture fluid on evaluating embryo quality and pregnancy outcome before implantation. Methods: 330 specimens of embryos culture fluid were selected as the study group before vitro fertilization and embryo transfer( IVF/ICSI),and the study group was divided into IL-8 positive group( IL-8 > 0. 19 ng/L) and IL-8 negative group( IL-8≤0. 19 ng/L). The culture fluid without embryo was selected as negative control in 9 cases. The content of IL-8 in culture fluid was determined by the technique of liquid chip high throughput protein analysis( LHTPA); the levels of IL-8 in the embryo culture fluid of the negative control group,the IL-8 positive group and the IL-8 negative group were compared; The age,duration of infertility,cause of disease( factors of primary infertility,secondary infertility and oviduct),the number of embryo transfer,the number of retrieved oocytes,the number of transferred embryos and the rate of missed pregnancy were between the IL-8 positive group and the IL-8 negative group. IL-8 positive and negative components as well as pregnancy rates were compared between normal embryos and high quality embryo transfer recipients. The total number of patients receiving embryo transfer( NLBPP) was compared with the pregnancy rate,clinical pregnancy rate,implantation rate,biochemical pregnancy rate,abortion rate,live birth rate and the number of live birth in IL-8 positive and negative group.Result: The positive rate of IL-8 in the study group was 32. 42%; the level of IL-8 in IL-8 positive group was higher than that in negative control group and IL-8 negative group( P < 0. 05); the age,duration of infertility,etiology( primary infertility and secondary infertility),embryo transfer cycles,endometrial thickness on the day of HCG injection,the number of oocytes,missed visit rate in pregnancy of examinee were compared in IL-8 positive and negative group; the difference was not statistically significant( P > 0. 05). Constituent ratio of transplanted high-quality or common embryos was compared in IL-8 positive and negative group,and the difference was not statistically significant( P = 0. 76). The pregnancy rate of transplanted high-quality embryos in IL-8 positive group was higher than that of IL-8 negative group( P = 0. 02),and the difference was not statistically significant( P = 0. 82). The rate of pregnancy and embryo implantation in the IL-8 positive group was significantly higher than that in the IL-8 negative group( P < 0. 05),and the difference was not statistically significant in other indexes( P= 0. 05 or > 0. 05). Conclusion: IL-8 can be used as an index to evaluate the developmental potential of embryos in embryo culture fluid before implantation.

关键词(KeyWords): 胚胎;移植;妊娠结局;白细胞介素-8;试管婴儿;体外受精-胚胎移植;液相芯片高通量蛋白质分析
embryo;transplant;pregnancy outcome;interleukin-8;test tube baby;in vitro fertilization-embryo transplantation;liquid chip high throughput protein analysis

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(86547321);; 贵州省科学技术基金[黔科合J字(2013)2056号];; 贵州医科大学附属医院博士科研启动基金资助

作者(Author): 黄官友;龙爱专;赵淑云;周桦;杨朝;田维婷;
HUANG Guanyou;LONG Aizhuan;ZHAO Shuyun;ZHOU Hua;YANG Chao;TIAN Weiting;Reproductive Medicine Center of Affiliated Hospital of Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2017.11.011

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