贵州医科大学学报

2004, (05) 395-399

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丹芍化纤胶囊对基质金属蛋白酶-1表达的影响
Effects of Danshaohuaxian Capsules on Expression of MMP-1 in Rat Hepatic Fibrosis

谢汝佳,杨勤,罗新华,耿晓霞,韩冰,李诚秀,程明亮
XIE Ru-jia 1, YANG Qin 1, LUO Xin-hua 2, GENG Xiao-xia 3, HAN Bing 1, LI Cheng-xiu 4, CHENG Ming-liang 3 (1. Department of Pathophysiology, Guiyang Medical College, Guiyang 550004, China; 2. Department of Lemology, Guiyang Medical College, Guiyang 550004, China; 3. Department of Lemology Guizhou Provincial People's Hospital, Guiyang 550002, China; 4. Department of Pharmacology, Guiyang Medical College, Guiyang 550004, China)

摘要(Abstract):

目的 :探讨丹芍化纤胶囊抗大鼠肝纤维化的作用及对基质金属蛋白酶 1(MMP 1)表达的影响。方法 :雄性Wistar大鼠 80只分为正常组、肝纤维化模型组、自然恢复组、低剂量治疗组、高剂量治疗组 ,除正常组外 ,其余采用四氯化碳 (CCl4)、饮酒、高脂低蛋白饮食等复合病因刺激制备肝纤维化动物模型 8周 ,然后两治疗组分别予以低剂量 (0 5g/kg)、高剂量 (1g/kg)丹芍化纤胶囊灌胃 8周。实验结束后测定肝脏指数、血清透明质酸(HA)及谷丙转氨酶 (ALT) ,光镜下观察肝组织纤维化程度 ,检测尿羟脯氨酸 (Hyp)排出量 ,同时免疫组织化学SABC法检测肝组织MMP 1的表达。结果 :治疗组与肝纤维化模型组及自然恢复组比较 ,肝脏指数、血清HA及ALT显著下降 ,肝纤维化程度显著减轻 ,尿Hyp排出明显增加 ,肝脏MMP 1表达显著增加。以上结果均以高剂量组较低剂量组改善明显。结论 :丹芍化纤胶囊增加大鼠肝组织中MMP 1的表达 ,可能是其抗纤维化作用的机制之一。
Objective: To investigate the effects of Danshaohuacian (DSHX) capsules which is based on traditional Chinese medicine prescription, on the expression of MMP-1 in rat hepatic fibrosis.Methods: 80 male Wistar rats were randomly divided into normal control group (CG), CCl 4-induced hepatic fibrosis group (CIG), non-DSHX-treated group (NDG), low-dose-treated group (LDG) and high-dose-treated group (HDG). Except for the normal control group, the rats were subcutaneously injected with CCl 4, given alcohol drinking, diet with high lipid and low protein for 8 weeks to produce hepatic fibrotic models. Then, rats in groups LDG and HDG were treated respectively with low dose (0.5 g/kg) and high dose (1.0 g/kg) of DSHX capsule by p.o everyday for 8 weeks. At the end of the experiment, the liver indexes were calculated; the serum hyaluronic acid (HA) and alanine aminotransferase (ALT) were examined; the degree of hepatic fibrosis were evaluated with optical microscopy; the urinary excretion of hydroxyproline (Hyp) was determined; the expression of collagen type I, III were detected with immunohistochemical techniques.Results: Compared with the indexes of hepatic fibrosis group and non-DSHX-treated group, it was revealed that in the two treated groups: the liver indexs, levels of serum HA and ALT and stage of hepatic fibrosis were all significantly reduced; the urinary excretion of Hyp increased; the expression of MMP-1 in liver tissues increased. All these alterations in HDG group were more obvious than those in LDG group,but no difference was found.Conclusion: The DSHX capsule could enhance the expression of MMP-1 in the liver tissues of CCl 4-induced hepatic fibrosis rats, which might be related with its anti-hepatic fibrosis activity.

关键词(KeyWords): 四氯化碳;肝硬化;间质胶原酶;羟脯氨酸;大鼠;丹芍化纤胶囊
carbon tetrachloride; liver cirrhosis; interstitial collagenase; hydroxyproline; rats; Danshaohuaxian capsule

Abstract:

Keywords:

基金项目(Foundation): 贵州省特殊人才基金资助( 2 0 0 3 1 )

作者(Author): 谢汝佳,杨勤,罗新华,耿晓霞,韩冰,李诚秀,程明亮
XIE Ru-jia 1, YANG Qin 1, LUO Xin-hua 2, GENG Xiao-xia 3, HAN Bing 1, LI Cheng-xiu 4, CHENG Ming-liang 3 (1. Department of Pathophysiology, Guiyang Medical College, Guiyang 550004, China; 2. Department of Lemology, Guiyang Medical College, Guiyang 550004, China; 3. Department of Lemology Guizhou Provincial People's Hospital, Guiyang 550002, China; 4. Department of Pharmacology, Guiyang Medical College, Guiyang 550004, China)

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DOI: 10.19367/j.cnki.1000-2707.2004.05.006

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