贵州医科大学学报

2016, v.41;No.188(05) 543-545

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

血清循环DNA定量检测在卵巢癌诊断中的应用价值
Clinical Application Value of Quantitative Detection of Serum Circulating DNA in Diagnosis of Ovarian Cancer

刘丽荣;文春蓉;梁璐;刘咏梅;朱丽英;
LIU Lirong;WEN Chunrong;LIANG Lu;LIU Yongmei;ZHU Liying;Department of Clinical Hematology,the College of Clinical Laboratory Science,Guizhou Medical University;Department of Clinical Laboratory,Guiyang Women and Children Hospital;

摘要(Abstract):

目的:探讨血清循环DNA(cDNA)定量检测在卵巢癌患者诊断中的应用价值。方法:以252例经病理证实的上皮性卵巢癌患者为卵巢癌组,100例卵巢良性肿瘤患者作为良性对照组,健康体检者100例作为健康对照,以微量基因组抽提试剂盒抽提3组患者血清DNA,用实时荧光定量Real-time PCR测定其含量。结果:良性对照组和健康对照组相比,血清cDNA比值差异无统计学意义(P>0.05);与良性对照组和健康对照组相比,卵巢癌组血清cDNA Ct值减少,差异有统计学意义(P<0.05);上皮性卵巢癌Ⅰ、Ⅱ、Ⅲ期血清cDNA Ct值差异无统计学意义(P>0.05),但Ⅳ期与Ⅰ期比较,差异有统计学意义(P<0.05)。结论:定量检测上皮性卵巢癌患者血清cDNA,有望成为临床辅助诊断卵巢癌的新手段。
Objective: To explore application value of quantitative detection of serum circulating DNA( cDNA) in diagnosing ovarian cancer. Methods: 252 cases of pathologically confirmed patients with epithelial ovarian cancer were enrolled as ovarian cancer group,100 cases of patients with benign ovarian cancer as benign control group,and 100 healthy people as healthy control group. Micro-genomicDNA extraction kit was adopted to extract the serum circulating DNA from the three groups and Real-time fluorescence quantitative PCR was adopted to detect the serum cDNA level. Results: There was no significantly statistical difference in level of serum cDNA between benign control group and healthy control group. There was significantly statistical difference in level of serum cDNA between ovarian cancer group and benign control group or between ovarian cancer group and healthy control group( P < 0. 05). In different clinical stage epithelial ovarian cancer,there was no difference in level of serum cDNA. Conclusion: Quantitative detection of serum cDNA of patients with ovarian cancer might be a novel auxiliary method for diagnosis of ovarian cancer.

关键词(KeyWords): 血清学;DNA;卵巢肿瘤;诊断技术与方法
serology;DNA;ovarian cancer;diagnosis technique and method

Abstract:

Keywords:

基金项目(Foundation): 贵州省科学技术基金[黔科合J字(2011)2233号]

作者(Author): 刘丽荣;文春蓉;梁璐;刘咏梅;朱丽英;
LIU Lirong;WEN Chunrong;LIANG Lu;LIU Yongmei;ZHU Liying;Department of Clinical Hematology,the College of Clinical Laboratory Science,Guizhou Medical University;Department of Clinical Laboratory,Guiyang Women and Children Hospital;

Email:

DOI: 10.19367/j.cnki.1000-2707.2016.05.012

文章评论(Comment):

序号(No.) 时间(Time) 反馈人(User) 邮箱(Email) 标题(Title) 内容(Content)
反馈人(User) 邮箱地址(Email)
反馈标题(Title)
反馈内容(Content)
扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享