贵州医科大学学报

2007, No.128(05) 484-487+491

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原因不明习惯性流产患者的Th亚群激活状态研究
A Study on Active Status of Lymphocyte Subset Thin Unexplained Recurrent Abortion Patients

谭玉洁;马莉;王焰;孟荣琼;刘若英;
TAN Yujie1,MA Li1,WANG Yan1,MENG Rongqiong2,LIU Ruoying3(1.The Central Clinical Laboratory,The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,China;2.Department of Obstetrics and Gynecology,The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,China;3.Department of Clinical Laboratory Science,Guiyang Medical College,Guiyang 550004,China)

摘要(Abstract):

目的:研究原因不明习惯性流产(URA)患者的TORCH活动性感染率、淋巴细胞亚群及Th1/Th2细胞激活状态,以探讨与URA有关的发病机制。方法:用流式细胞术计数外周血淋巴细胞及其亚群;流式细胞仪结合微珠捕获法(Cytometric Bead Array,CBA)测定血清中6种Th1/Th2源细胞因子水平,酶联免疫吸附法(ELISA)检测血清中的TORCH抗体IgM和IgG。结果:(1)URA组的HSVⅡ-IgG和CMV-IgG的阳性率(46.2%,59.2%)分别高于对照组的阳性率(24.0%和44.8%),差异具有显著性意义(P<0.05);URA组的IgM类抗体阳性率全部高于对照组,差异均具有极显著性意义(P<0.01)。(2)URA患者外周血T细胞数:(62.9±7.2)%,较对照组(67.9±8.4)%减少有显著性意义(P<0.05);NK细胞数(16.3±8.4),较对照组(12.4±3.1)%增加有显著性意义(P<0.05)。(3)URA患者IL-2(6.1±1.6)ng/L及IL-5(4.5±1.4)ng/L较对照组(5.0±1.2)ng/L及(2.7±0.6)ng/L升高有显著性意义(P<0.05)。结论:(1)URA患者的活动性TORCH感染率显著升高;(2)URA的发生与该类患者T细胞总数减少以及NK细胞数量增加存在一定关联;(3)URA患者的Th1、Th2亚群均似有激活。
Objective: To investigate the role of infection with Toxoplasma gondii,rubella virus,cytomegalovirus(CMV) and herpersimples virus(TORCH),and lymphocyte subsets and TH1/TH2 active status in unexplained recurrent abortion(URA),and so as to probe into the principle of the diseases.Methods: The number of immunity cells and the active subsets in whole blood were counted with flow cytomter(FCM).Cytometric bead array(CBA) was used to determine serum levels of cytokines derived from Th1/Th2 helper lymphocyte,and ELISA was used to determine antibody IgM and IgG against TORCH in serum.Results: (1) The positive rates of HSVⅡ-IgG and CMV-IgG in URA patients were 46.2% and 59.2% repectively,which were higher significantly than those in controls(24.0%,44.8%)(P<0.05).The positive rates of four kinds of IgM were all higher significantly than those in control group(P<0.01);(2) Compared with control group(67.9±8.4,12.4±3.1),URA patients' CD3+cell(62.9±7.2)fell and CD16+56+cell(16.3±8.4) rose significantly(P<0.05);(3) In URA patients,serum levels of IL-2(6.1±1.6)ng/L and IL-5(4.5±1.4)ng/L were higher than those in controls(5.0±1.2)ng/L,(2.7±0.6)ng/L(P<0.05).Conclusions:(1)The active infection rates of TORCH in URA patients are increased;(2) URA is connected with falling of total T cell numbers and rising of NK cell numbers;(3) Both subsets Th1 and Th2 seem to be activated in URA patients.

关键词(KeyWords): 流产,习惯性;感染;淋巴细胞亚群;细胞因子类;流式细胞术;Th源细胞因子
abortion,habitual;infection;lymphocyte subsets;cytokines;flow cytometry;Th-derived CK

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作者(Author): 谭玉洁;马莉;王焰;孟荣琼;刘若英;
TAN Yujie1,MA Li1,WANG Yan1,MENG Rongqiong2,LIU Ruoying3(1.The Central Clinical Laboratory,The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,China;2.Department of Obstetrics and Gynecology,The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,China;3.Department of Clinical Laboratory Science,Guiyang Medical College,Guiyang 550004,China)

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DOI: 10.19367/j.cnki.1000-2707.2007.05.012

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