贵州医科大学学报

2018, v.43;No.211(04) 418-421

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孕哺期染氟对子代大鼠空间记忆及中枢单胺类神经递质的影响
Effects of Fluoride Exposure during Pregnancy on Spatial Memory and Central Monoamine Neurotransmitters in Offspring Rats

张文均;刘南暖;
ZHANG Wenjun;LIU Nannuan;Department of Neurology,Affiliated People's Hospital of Hubei Medical College;

摘要(Abstract):

目的:探讨雌鼠孕哺期染氟对子代空间记忆能力及中枢单胺类神经递质的影响。方法:健康8周龄Wistar雌性妊娠大鼠24只,随机分为对照组、高氟染毒组和低氟染毒组,对照组给予蒸馏水喂养,高氟染毒组自妊娠开始至哺乳期(产仔鼠后6周)给予20 mg/kg氟化钠溶液,低氟染毒组自妊娠开始至哺乳期(产仔鼠后6周)给予5 mg/kg氟化钠溶液;记录仔鼠出生后1、3及6周时的体重;造模结束时各组仔鼠进行Morris水迷宫定位航行实验记录逃避潜伏期评价空间记忆能力,水迷宫实验结束后取鼠海马组织,荧光分光光度法检测海马组织匀浆中单胺类神经递质甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT),黄嘌呤氧化酶法检测仔鼠脑组织超氧化物歧化酶(SOD),硫代巴比妥酸法检测仔鼠脑组织丙二醛(MDA)。结果:高氟染毒组第1、2、3、4天时的逃避潜伏期均长于对照组,差异有统计学意义(P<0.05);高氟染毒组仔鼠6周时体重明显低于对照组、海马组织匀浆中单胺类神经递质NE、DA、5-HT明显低于对照组,脑组织中SOD明显低于对照组和低氟染毒组,MDA明显高于对照组和低氟染毒组,差异有统计学意义(P<0.05);低氟染毒组仔鼠脑组织中SOD明显低于对照组,MDA明显高于对照组,差异有统计学意义(P<0.05)。结论:孕哺期高氟染毒降低了仔鼠海马中单胺类神经递质NE、DA和5-HT含量,影响了仔鼠空间记忆能力。
Objective: To explore the effects of fluoride exposure during pregnancy on spatial memory and central monoamine neurotransmitters in offspring rats. Methods: 24 healthy 8-week old Wistar female pregnant rats were randomly divided into the control group,the high fluorine dye group and the low fluorine dye group. The control group was fed with distilled water; the high fluoride group received20 mg/kg sodium fluoride solution from pregnancy to lactation( 6 weeks after birth); the low fluoride group received 5 mg/kg sodium fluoride solution from the beginning of pregnancy to the lactation period( 6 weeks after birth); body weight of postnatal rats at 3 and 6 weeks after birth was recorded. Morris water maze navigation experiment recording the evaluation of spatial memory ability by escape latency was conducted in rats of each group after molding. After the water maze experiment,the hippocampal tissue was taken. Monoamine neurotransmitter norepinephrine in hippocampus homogenate( NE),dopamine( DA) and 5-HOT were detected by fluorescence spectrophotometry; superoxide dismutase in brain tissue of newborn was detected by the method of xanthine oxidase; malondialdehyde in brain tissue of newborn was detected by thiobarbituric acid method. Results: The escape latency of the high fluoride exposure group was longer than that of the control group on the first,second,third and fourth day,and the difference was statistically significant( P < 0. 05). The body weight of rats exposed to high fluoride at 6 weeks was significantly lower than that of the control group; the NE,DA and 5-HT levels of monoamine neurotransmitters in the homogenate of hippocampus were significantly lower than those in the control group; SOD in brain tissue was significantly lower than that in control group and low fluoride exposure group; MDA was significantly higher than that of control group and low fluoride exposure group,and the difference was statistically significant( P < 0. 05). The SOD in brain tissue of rats exposed to low fluoride was significantly lower than that of control group,and the difference was statistically significant( P < 0. 05). Conclusion: The concentration of monoamine neurotransmitter NE,DA and 5-HT in hippocampus of neonatal rats is decreased by high fluoride exposure during pregnancy,which affects the spatial memory ability of rats.

关键词(KeyWords): 染氟;孕哺期;单胺类神经递质;空间记忆;大鼠,Wister
fluoride;pregnancy and lactation;monoamine neurotransmitter;spatial memory;rats,Wister

Abstract:

Keywords:

基金项目(Foundation): 十堰市科技局科技项目(14Y31)

作者(Author): 张文均;刘南暖;
ZHANG Wenjun;LIU Nannuan;Department of Neurology,Affiliated People's Hospital of Hubei Medical College;

Email:

DOI: 10.19367/j.cnki.1000-2707.2018.04.011

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