贵州医科大学学报

2017, v.42;No.201(06) 661-665

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某54度食用白酒对大鼠肝细胞TNF-α表达及凋亡的影响
Effects of Certain 54° Edible Liquor on TNF-α Expression and Apoptosis of Hepatocyte in Rats

王燕林;梁文妹;李容;陈兴强;
WANG Yanling;LIANG Wenmei;LI Rongrong;CHEN Xingqiang;Department of Histology and Embryology,Guizhou Medical University;

摘要(Abstract):

目的:探讨摄入某品牌54度食用白酒后,不同时长及剂量下,对大鼠肝细胞肿瘤坏鲜红因子(TNF-α)表达及凋亡的影响。方法:将115只正常雄性SD大鼠随机分为正常对照组(n=25)及实验组(n=90),实验组分为低剂量组[0.8 m L/(kg·d)]、中剂量组[1.6 m L/(kg·d)]、高剂量组[2.4 m L/(kg·d)];实验组大鼠每日上午11点及下午5点用该白酒各灌胃1次,正常组不予处理,分别于第4、8、12、16和20周末时取肝脏组织,采用免疫组化SABC法检测TNF-α表达,TUNEL法检测各组大鼠肝细胞凋亡、图像分析及形态计量法检测大鼠肝细胞TNF-α平均光密度值及凋亡指数(AI)。结果:正常对照组及各实验组肝细胞中,TNF-α免疫反应的阳性产物主要集中于胞浆,TUNEL检测肝细胞凋亡阳性产物存在于肝细胞核内,与正常对照组比较,第4周、8周、12周、16周及20周末时各实验组肝细胞内TNF-α免疫反应阳性产物位置及染色强度未见明显变化,AI也无明显改变,差异无统计学意义(P>0.05)。结论:本实验设定的剂量和时程内,用该白酒灌胃后,对大鼠肝细胞TNF-α表达水平及AI未见明显影响。
Objective: To investigate the effect of the expression of TNF-α and the apoptosis of rat hepatocytes after intake certain 54° edible liquor at different doses and schedules. Methods: 115 normal adult male SD rats were randomly divided into control group( n = 25) and an experimental group( n= 90). Each experimental group was further divided into low dose group[0. 8 m L/( kg·d) ],middle dose group[1. 6 m L/( kg·d) ],high dose group[2. 4 m L/( kg·d) ]. The experimental groups were fed by gavage twice daily( 11 ∶ 00 AM an 5 ∶ 00 PM) with the liquor,the control group received no treatment. At the end of the 4th,8th,12 th,16th and 20 th week,liver tissue was taken. The expression of TNF-α was detected by immunohistochemical SABC method; The apoptosis of hepatocyte was detected by terminal deoxynucleotidyl transferase mediated nick end labeling( TUNEL). The mean optical density of TNF-α and apoptosis index in rat hepatocytes were detected by image analysis and morphometry. Results: TNF-α immunohistochemical positive products existed in cytoplasm of hepatocyte;TUNEL test indicated hepatocyte apoptosis positive product exists in the nucleus; immunoreaction positive product location of hepatocyte TNF-α and the staining intensity in the experimental groups at the end of 4th,8th,12 th,16th,and 20 th week,has no obvious change,the same also applied to AI,differences were not statistically significantly( P > 0. 05). Conclusion: TNF-α expression level and metric measurement; alcoholic liver diseaseapoptotic index of hepatocyte in rat liver has no obvious change in this experiment at set doses and schedules after gavage with the liquor.

关键词(KeyWords): 肿瘤坏死因子;肝细胞;凋亡;免疫组织化学;形态计量;酒精性肝病
tumor necrosis factor alpha;hepatocytes;apoptosis;immunohistochemistry;morphometric measurement;alcoholic liver disease

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基金项目(Foundation): 贵州省教育厅“125”重大科技专项基金资助[黔教合重大专项字(2012)007]

作者(Author): 王燕林;梁文妹;李容;陈兴强;
WANG Yanling;LIANG Wenmei;LI Rongrong;CHEN Xingqiang;Department of Histology and Embryology,Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2017.06.009

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