家兔发育期5-羟色胺及嗜铬粒素A在胃窦黏膜中的表达Expression of 5-hydroxytryptamine and Chromogranin A in Gastric Antrum of Rabbit during Postnatal Development Period
夏白娟;梁文妹;洪艳;韩晶;胡赟;李一欣;
XIA Baijuan;LIANG Wenmei;HONG Yan;HAN Jing;HU Yun;LI Yixin;Department of Histology and Embryology,Guiyang Medical College;
摘要(Abstract):
目的:观察家兔生后发育期间5-羟色胺(5-HT)和嗜铬粒素A(CgA)在胃窦黏膜中的分布、形态和数量变化。方法:生后5、15、25、35、60及90 d家兔各10只,处死后取胃窦组织,用免疫组织化学SABC法和图像分析法观察5-HT、CgA免疫反应细胞的定位和表达。结果:不同发育阶段胃窦黏膜内均有5-HT和CgA的表达,阳性产物位于细胞胞质内;生后5 d和15 d时5-HT阳性细胞分散存在于黏膜上皮内,染色较浅,35 d开始逐渐增多,60 d时数量最多(P<0.05),90 d时减少至35 d水平,细胞平均灰度值于35 d时降低,60 d时达到最低(P<0.05);CgA阳性细胞从生后5 d开始数量逐渐增多,尤以90 d增多明显(P<0.05),平均灰度值于90 d时最低(P<0.05)。结论:家兔生后发育期间胃窦5-HT、CgA阳性细胞的分布和数量变化有一定的规律性,提示其所释放的激素可能参与家兔胃窦的发育和某些生理活动。
Objective: To study the distribution,and changes in morphology and number of 5hydroxytryptamine(5-HT) and chromogranin A(CgA) positive cells in gastric antrum during the postnatal development period.Methods: Sixty rabbits were divided into 6 groups according to their age which were 5d,15d,25d,35d,60d,and 90d respectively,with 10 rabbits in each group.They were executed and their gastric antrum tissues were taken,and embedded in routine paraffin and then made into serial sections.Immunohistochemical SABC staining and image analysis were performed to detect the expressions and the localization of 5-HT and CgA immunoreactive cells.Results: The expression of 5-HT and CgA was found at different postnatal development stages,and the positive substances were located in plasma.In the 5d and 15d groups,a few 5-HT positive cells were scattered in the mucosa epithelium and their staining was weak.From the 35thday,the number of 5-HT positive cells was ascendent and obviously reached a peak on the 60thday(P < 0.05),The mean grey value of 5-HT positive cells decreased from the 35thday and reached the lowest on the 60thday(P < 0.05).The number of CgA positive cells gradually increased from the 5thday,and the change was getting prominent especially on the 90thday(P < 0.05).The mean grey value of those was lowest on the 90thday(P < 0.05).Conclusions: The distribution and the number changes of 5-HT and CgA positive cells follow certain regularity,which suggests that the hormones secreted by 5-HT and CgA positive cells may be associated with the development and physiologic function of rabbit.
关键词(KeyWords):
5-羟色胺;嗜铬粒素A;生后发育;幽门窦;兔
5-hydroxytryptamine;chromogranin A;postnatal development;pyloric antrum;rabbit
基金项目(Foundation):
作者(Author):
夏白娟;梁文妹;洪艳;韩晶;胡赟;李一欣;
XIA Baijuan;LIANG Wenmei;HONG Yan;HAN Jing;HU Yun;LI Yixin;Department of Histology and Embryology,Guiyang Medical College;
Email:
DOI: 10.19367/j.cnki.1000-2707.2013.05.018
参考文献(References):
- [1]徐俊荣,罗金燕.功能性消化不良研究的新概念[J].国际消化病杂志,2007(6):426-427,430.
- [2]胡赟,梁文妹.胰岛淀粉样多肽在生后发育期间家兔十二指肠中的表达及意义[J].世界华人消化杂志,2009(9):910-913.
- [3]张素娟,王彤,王东旭,等.小鼠生后发育期间胃肠道5-羟色胺及生长抑素免疫反应细胞的形态学观察[J].中国医药导报,2009(29):7-10.
- [4]Watts SW,Thompson JM.Characterization of the con-tractile 5-hydroxytryptamine receptor in the renal artery ofthe normotensive rat[J].J Pharmacol Exp Ther,2004(1):165-172.
- [5]赵素贤,王秀琴,杜鹃,等.大鼠脾气虚结合胃溃疡模型中5-羟色胺及其受体的变化[J].中国组织化学与细胞化学杂志,2006(5):499-503.
- [6]Khan WI,Ghia JE.Gut hormones:emerging role in im-mune activation and inflammation[J].Clin Exp Immu-nol,2010(1):19-27.
- [7]Portela-Gomes GM,Stridsberg M.Chromogranin A in thehuman gastrointestinal tract:an immunocytochemicalstudy with region-specific antibodies[J].J HistochemCytochem,2002(11):1487-1492.
- [8]Gibson CJ,Munoz DG.Chromogranin A inhibits dopa-mine release from rat striatal slices[J].J Neural Transm,1998(10-12):1083-1089.
- [9]Singh S,Law C.Chromogranin A:a sensitive biomarkerfor the detection and post-treatment monitoring of gastro-enteropancreaticneuroendocrine tumors[J].Expert RevGastroenterol Hepatol,2012(3):313-334.
- [10]Chu S,Schubert ML.Gastric secretion[J].Curr OpinGastroenterol,2012(6):587-593.
- [11]陈娟,葛振华,周凡,等.幽门螺杆菌感染对慢性胃炎胃窦部EC细胞和CgA细胞的影响[J].中国组织化学与细胞化学杂志,2008(5):465-469.
- [12]王海萍,任君旭,张雷,等.大鼠实验性胃溃疡自愈期间肾上腺髓质神经肽Y、5羟色胺及嗜铬颗粒素A免疫阳性细胞的变化[J].解剖学杂志,2007(4):420-423.
文章评论(Comment):
|
||||||||||||||||||
|
- 夏白娟
- 梁文妹
- 洪艳
- 韩晶
- 胡赟
- 李一欣
XIA Baijuan- LIANG Wenmei
- HONG Yan
- HAN Jing
- HU Yun
- LI Yixin
- Department of Histology and Embryology
- Guiyang Medical College
- 夏白娟
- 梁文妹
- 洪艳
- 韩晶
- 胡赟
- 李一欣
XIA Baijuan- LIANG Wenmei
- HONG Yan
- HAN Jing
- HU Yun
- LI Yixin
- Department of Histology and Embryology
- Guiyang Medical College