杨秀海;高晓勤;李源;陈乾美;
YANG Xiuhai1,GAO Xiaoqin2,LI Yuan3,CHEN Qianmei1(1.Department of Otorhinolarynglogy,The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,China;2.Department of Histo-Embryology,Guiyang Medical College,Guiyang 550004,China;3.Department of Otorhinolarynglogy-Head and Cerv Surgery,The Third Affiliated Hospital of Yat-sen University, Guangzhou 510080,Guangdong,China)

• 1:贵阳医学院附院耳鼻咽喉-头颈外科
• 2:贵阳医学院组织学与胚胎学教研室
• 3:中山大学附属三院耳鼻咽喉-头颈外科
• 4:贵阳医学院附院耳鼻咽喉-头颈外科 贵州贵阳550004

摘要(Abstract)：

目的:探讨Toll样受体4(Toll like receptor 4,TLR4)mRNA及人β防御素2(human beta-defensin-2,HBD-2)在鼻黏膜组织中的表达及意义。方法:采用核酸分子原位杂交技术和免疫组织化学技术分别检测慢性鼻、鼻窦炎患者鼻黏膜组织中TLR4 mRNA和HBD-2的表达。结果:TLR4 mRNA在病变组鼻黏膜组织中的表达明显强于对照组(P<0.05)。但在病变组中13例Ⅰ型和17例Ⅱ型慢性鼻、鼻窦炎患者的病变鼻黏膜组织中的表达差异无显著性;HBD-2在对照组未见表达,而在病变组均出现不同程度的表达,两组间的表达差异具有显著性(P<0.01),而且在Ⅱ型病变鼻黏膜组织中的表达明显强于Ⅰ型(P<0.05)。结论:TLR4可能参与了鼻黏膜天然免疫,HBD-2可能是炎症反应的重要产物之一。致炎因子通过TLR4对病原微生物进行识别,激活鼻黏膜上皮细胞,最终导致炎性细胞产生大量的HBD-2,这可能在鼻黏膜启动有效的炎性免疫反应过程中具有一定的作用。
Objective: To investgate the expression and significance of TLR4 mRNA and HBD-2 in human nasal mucosa.Methods: Specimens from 30 patients with chronic rhinosinusitis and 15 noraml adults were examined by in situ hybridization for TLR4 mRNA and by immunohistochemistry for HBD-2.Results: All of the 30 samples of chronic rhinosinusitis showed a stronger expression of TLR4 mRNA(P<0.05) and HBD-2(P<0.01) than those of noraml adults.Conclusion: Epithelia in human nasal mucosa could present a novel defense mechanism during bacterial infections with the help of TLR4,and HBD-2 might be the product of the process.

关键词(KeyWords)： 鼻黏膜;β防御素;免疫组织化学;Toll样受体4;人β防御素2;核酸分子原位杂交技术
nasal mucosa;beta-defensins;immunohistochemistry;toll like receptor 4;human beta-defensin-2;in situ hybridization

Abstract:

Keywords:

基金项目(Foundation): 贵州省省长基金资助项目(20052A357)

作者(Author): 杨秀海;高晓勤;李源;陈乾美;
YANG Xiuhai1,GAO Xiaoqin2,LI Yuan3,CHEN Qianmei1(1.Department of Otorhinolarynglogy,The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,China;2.Department of Histo-Embryology,Guiyang Medical College,Guiyang 550004,China;3.Department of Otorhinolarynglogy-Head and Cerv Surgery,The Third Affiliated Hospital of Yat-sen University, Guangzhou 510080,Guangdong,China)

Email:

DOI: 10.19367/j.cnki.1000-2707.2007.05.004

参考文献(References)：

• [1]Merino LA,Ronconi MC,Hrenuk GE,et al.Bacteriologicfindings in patients with chronic sinusitis[J].Prim Care,2003(1):137-154.
• [2]左可军,史剑波,樊韵平,等.慢性鼻窦炎的细菌学研究[J].中华耳鼻咽喉头颈外科杂志,2005(7):524-527.
• [3]Bhattacharyya N,Kepnes L J.The microbiology of recur-rent rhinosinusitis after endoscope in sinus surgery[J].Arch Otol Head Neck Surg,1999(125):1117-1120.
• [4]李艳妮,刘邦华,孔维佳.鼻内镜鼻窦手术后迁延性鼻窦炎的细菌学研究[J].临床耳鼻咽喉科杂志,2005(24):1131-1133.
• [5]王成硕,董震.Toll样受体mRNA在慢性鼻窦炎患者鼻黏膜上皮细胞中的表达[J].中华耳鼻咽喉科杂志,2003(38):243-246.
• [6]Pitzurra L,Bellocchio S,Nocentini A,et al.Antifungalimmune reactivity in nasal polyposis[J].Infect Immun,2004(12):775-781.
• [7]Diamond G,Kaiser V,Rhodes J,et al.Transcriptional reg-ulation of beta defensin gene expression in tracheal epithe-lial cells[J].Infect Immun,2000(1):113-119.
• [8]Masuhiro NISHIMURA,Shinsaku NAITO.Tissue-SpecificmRNA Expression Profiles of Human Toll-Like Receptorsand Related Genes[J].Biol Pharm Bull,2005(5):886-892.
• [9]Yarovinsky F,Zhang D,Andersen JF,et al.TLR11 activa-tion of dendritic cells by a protozoan profilin-like protein[J].Science,2005(5728):1626-1629.
• [10]李立斌,朱红.Toll样受体4信号转导研究进展[J].国外医学.生理、病理科学与临床分册,2005(2):162-166.
• [11]Gaicia JR,Krause A,Schulz S,et al.Human beta defensin2:a novel inducible peptide with a specific salt sensitivespectrum of antimicrobial activity[J].FASEB J,2001(15):1819-1821.
• [12]Ashitani J,Mukae H,Hiratsuka T,et al.Plasma and BAL fluid concentrations of antimicrobial peptides in pa-tients with Mycobacterium avium intracellular infection[J].Chest,2001(119):1131-1137.
• [13]田翠环.β2防御素与呼吸系统的防御和损伤[J].国外医学呼吸系统分册,2004(3):150-156.
• [14]Lee SH,Kim JE,Lim HH,et al.Antimicrobial defensinpeptides of the human nasal mucosa[J].Ann Otol RhinolLaryngol,2002(111):135-141.
• [15]杨秀海,李源,王振霖.内毒素刺激前后正常鼻黏膜上皮细胞中Toll样受体4mRNA、核因子кBP50亚型mR-NA的表达及意义[J].中华耳鼻咽喉头颈外科杂志,2006(9):698-701.

文章评论(Comment)：