何筑;况时祥;张树森;邹家莉;
HE Zhu;KUANG Shixiang;ZHANG Shusen;ZOU Jiali;Guiyang College of Traditional Chinese Medicine;The Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine;

• 1:贵阳中医学院研究生院
• 2:贵阳中医学院第二附属医院

摘要(Abstract)：

目的:探讨脑通汤对缺氧-复氧大鼠海马神经元脑源性神经营养因子基因(BDNF mRNA)表达的影响。方法:将体外培养大鼠海马神经元分为正常细胞组、缺氧-复氧模型组、正常血清组和脑通汤大、中、小剂量含药血清组,实时荧光定量PCR法检测脑通汤干预缺氧24 h复氧2 h后海马神经元BDNF mRNA的表达。结果:实时荧光定量PCR结果显示,与正常海马细胞组比较,缺氧-复氧模型组海马BDNF mRNA的相对表达量明显下降(P<0.05);与缺氧-复氧模型组海马细胞相比,脑通汤大、中、小剂量含药血清组海马细胞中BDNF mRNA的相对表达量明显增高(P<0.05),其中脑通汤大、中剂量含药血清组升高更明显(P<0.05);但正常血清组与模型组海马细胞比较BDNF mRNA的表达趋势差异无统计学意义(P>0.05)。结论:脑通汤可以上调缺氧-复氧后海马神经元BDNF mRNA的表达。
Objective: To investigate the effect of Naotong soup on the expression of brain-derived neurotrophic factor( BDNF) mRNA in hypoxia-reoxygenation rat hippocampal neurons. Methods:Hippocampal neurons of rats were cultured in vitro,then divided into normal cell group,hypoxia-reoxygenation model group,normal serum group,high,medium and low dosage drug containing Naotong soup group. The expression of BDNF mRNA in rat hippocampal neurons after hypoxia 24 h and reoxygenation 2 h was detected in real-time quantitative PCR. Results: Real-time fluorescent quantitative PCR indicated that comparing with normal cell group,the relative expression levels of BDNF mRNA decreased significantly in model group( P < 0. 05). Comparing with the model group,The relative expression levels of BDNF mRNA increased significantly in Naotong soup groups,meanwhile high and medium doses of Naotong soup groups were significantly higher( P < 0. 05). However,normal serum group and the model group showed no significant difference comparing BDNF mRNA expression trend( P > 0. 05). Conclusions: Naotong soup can improve the expression of BDNF mRNA in rat hippocampal neurons after hypoxia-reoxygenation.

关键词(KeyWords)： 复方(中药);脑通汤;缺氧;神经元;脑源性神经营养因子
composite(TCD);Naotong soup;anoxia;neurons;brain-derived neurotrophic factor

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81160490)

作者(Author): 何筑;况时祥;张树森;邹家莉;
HE Zhu;KUANG Shixiang;ZHANG Shusen;ZOU Jiali;Guiyang College of Traditional Chinese Medicine;The Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine;

Email:

DOI: 10.19367/j.cnki.1000-2707.2015.12.006

参考文献(References)：

• [1]Aggarwal NT,Decarli.Vascular dementia:emerging trends[J].Semin Neurol,2007(1):66-77.
• [2]刘国政.缺血性脑血管病的研究进展[J].实用心脑肺血管病杂志,2013(8):5-6.
• [3]林岚,苏美仙.脑缺血/再灌注损伤机制的研究现状[J].辽宁医学杂志,2013(1):20-22.
• [4]Barde YA.Trophic factor and neuronal survival[J].Neuron,1989(6):1525-34.
• [5]鲁丽敏,李艳,姜宇宙.脑源性神经营养因子与缺血性脑血管病的研究进展[J].局解手术学杂志,2005(4):269-270.
• [6]王丽晔,赵海苹,王荣亮,等.络瘀通胶囊对大鼠脑缺血-再灌注损伤的作用[J].中国脑血管病杂志,2014(12):650-655.
• [7]马贤德,王守岩,张威,等.眼针对脑缺血再灌注模型大鼠脑组织BDNF、p75NTR表达影响及其脑保护作用机制[J].北京中医药大学学报,2014(1):48-52.
• [8]宋先红,况时祥.脑通汤对血管性痴呆大鼠海马神经元凋亡基因Bcl-2、Bax及Bcl-2/Bax比例变化的影响[J].时珍国医国药,2014(11):2567-2570.
• [9]徐叔云,卞如濂,陈修.药理实验方法学[M].3版.北京:人民卫生出版社,2005:203.
• [10]邹移海,徐志伟,苏钢强.实验动物学[M].北京:科学出版社,2004:184.
• [11]Bossenmeyer PC.Kozie l V,Daval JL.Hypoxia/reoxygenation induceapop-tosis through biphasic induction of protein synthesis in cultured rat brain neurons[J].Neuroscience,2000(4):1157-65.
• [12]朱明瑾,唐启盛,李小黎,等.颐脑解郁方对海马神经元缺氧/复氧损伤突触素表达的影响[J].中华中医药杂志,2013(5):1547-1551.
• [13]Schmittgen TD,Livak KJ.Analyzing real-time PCR data by the compa-rative CT method[J].Nature Protocols,2008(3):1101-1108.
• [14]石苗茜,刘卫平.血管性痴呆发病机制研究进展[J].第四军医大学报,2007(9):4.
• [15]姜海鹏,况时祥.脑通胶囊治疗血管性痴呆早期疗效观察[J].中国中医急诊,2010(10):1669-1678.
• [16]张树森,况时祥,肖雁,等.脑通胶囊对VD大鼠海马组织NMDA受体1亚基和2B亚基mRNA表达的影响[J].中国老年学杂志,2012(32):79-82.
• [17]张树森,况时祥,肖雁,等.脑通胶囊对VD大鼠海马NMDA受体mRNA表达及锥体细胞的影响[J].中国实验方剂学杂志,2012(12):230-235.
• [18]Ascano M,Bodmer D,Kuruvilla R.Endocytic trafficking of neurotrophins in neuraldevelopment[J].Trends Cell Biol,2012(22):266-273.
• [19]Shakhbazau A,Martinez JA,Xu QG,et al.Evidence for a systemic regu-lation of neurotrophin synthesis in response to peripheral nerve injury[J].J Neurochem,2012(122):501-511.
• [20]Gordon T.The physiology of neural injury and regeneration:the role of neurotrophic factor[J].J Commun Disord,2010(43):265-273.
• [21]Connor B,Dragunow M.The role of neurotrophic growth factors in neu-rodegenerative disorders of the human[J].Brain Res Rev,1998(1):1-39.
• [22]Kim WS,Lim JY,Shin JH,et al.Effect of the presence of brain-derived Neurotrophic factor val(66)met po1ymorphism on the recovery in patients with acute subcortical stroke[J].Ann Rehabil Med,2013(3):311-319.
• [23]叶晓倩,江一静,游咏梅,等.电针对脑缺血再灌注型大鼠脑源性神经营养因子表达[J].中国康复医学,2014(3):204-207.
• [24]王强,张一,王榕,等.丹参多酚酸盐对缺血再灌注损伤大鼠脑组织BDNF和GDNF的影响[J].卒中与神经疾病,2013(5):272-274.
• [25]曲铁兵,俞天虹,刘志婷,等.补阳还五汤及其拆方对大鼠脑缺血后神经发生的影响[J],中国中西医结合杂志,2014(30):342-347.
• [26]王哲,王守岩,王德山,等.眼针对急性脑缺血再灌注损伤大鼠海马组织BDNF表达影响[J].针灸临床杂志,2011(7):57-60.
• [27]尹艳艳,李卫平,李维祖,等.黄芪总苷对大鼠局灶性脑缺血/再灌注损伤后BDNF、Trk B和p75NTRmRNA[J].中国药理学通报,2009(5):672-676.

文章评论(Comment)：