贵州医科大学学报

2015, v.40;No.183(12) 1306-1310

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

脑通汤对缺氧-复氧大鼠海马神经元BDNF mRNA表达的影响
Effect of Naotong Soup on the Expression of BDNF mRNA in Hypoxia-Reoxygenation Rat Hippocampal Neurons

何筑;况时祥;张树森;邹家莉;
HE Zhu;KUANG Shixiang;ZHANG Shusen;ZOU Jiali;Guiyang College of Traditional Chinese Medicine;The Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine;

摘要(Abstract):

目的:探讨脑通汤对缺氧-复氧大鼠海马神经元脑源性神经营养因子基因(BDNF mRNA)表达的影响。方法:将体外培养大鼠海马神经元分为正常细胞组、缺氧-复氧模型组、正常血清组和脑通汤大、中、小剂量含药血清组,实时荧光定量PCR法检测脑通汤干预缺氧24 h复氧2 h后海马神经元BDNF mRNA的表达。结果:实时荧光定量PCR结果显示,与正常海马细胞组比较,缺氧-复氧模型组海马BDNF mRNA的相对表达量明显下降(P<0.05);与缺氧-复氧模型组海马细胞相比,脑通汤大、中、小剂量含药血清组海马细胞中BDNF mRNA的相对表达量明显增高(P<0.05),其中脑通汤大、中剂量含药血清组升高更明显(P<0.05);但正常血清组与模型组海马细胞比较BDNF mRNA的表达趋势差异无统计学意义(P>0.05)。结论:脑通汤可以上调缺氧-复氧后海马神经元BDNF mRNA的表达。
Objective: To investigate the effect of Naotong soup on the expression of brain-derived neurotrophic factor( BDNF) mRNA in hypoxia-reoxygenation rat hippocampal neurons. Methods:Hippocampal neurons of rats were cultured in vitro,then divided into normal cell group,hypoxia-reoxygenation model group,normal serum group,high,medium and low dosage drug containing Naotong soup group. The expression of BDNF mRNA in rat hippocampal neurons after hypoxia 24 h and reoxygenation 2 h was detected in real-time quantitative PCR. Results: Real-time fluorescent quantitative PCR indicated that comparing with normal cell group,the relative expression levels of BDNF mRNA decreased significantly in model group( P < 0. 05). Comparing with the model group,The relative expression levels of BDNF mRNA increased significantly in Naotong soup groups,meanwhile high and medium doses of Naotong soup groups were significantly higher( P < 0. 05). However,normal serum group and the model group showed no significant difference comparing BDNF mRNA expression trend( P > 0. 05). Conclusions: Naotong soup can improve the expression of BDNF mRNA in rat hippocampal neurons after hypoxia-reoxygenation.

关键词(KeyWords): 复方(中药);脑通汤;缺氧;神经元;脑源性神经营养因子
composite(TCD);Naotong soup;anoxia;neurons;brain-derived neurotrophic factor

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81160490)

作者(Author): 何筑;况时祥;张树森;邹家莉;
HE Zhu;KUANG Shixiang;ZHANG Shusen;ZOU Jiali;Guiyang College of Traditional Chinese Medicine;The Second Affiliated Hospital of Guiyang College of Traditional Chinese Medicine;

Email:

DOI: 10.19367/j.cnki.1000-2707.2015.12.006

参考文献(References):

文章评论(Comment):

序号(No.) 时间(Time) 反馈人(User) 邮箱(Email) 标题(Title) 内容(Content)
反馈人(User) 邮箱地址(Email)
反馈标题(Title)
反馈内容(Content)
扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享