贵州医科大学学报

2015, v.40;No.178(07) 747-749+753

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5种尿沉渣染色方法的效果比较
Comparison of the Effects of 5 Urinary Sediment Staining Methods

严雪梅;刘宝;夏曙华;
YAN Xuemei;LIU Bao;XIA Shuhua;College of Life Science,Sichuan University;No.1 Affiliated Hospital of Guiyang College of Traditional Chinese Medicine;Departmet of Microbiology and Immunology,Affiliated Hospital of Guizhou Medical University;Institude of Clinical Laboratory,Guizhou Medical University;

摘要(Abstract):

目的:探讨5种尿沉渣染色法的最佳染色条件及效果。方法:180例尿沉渣异常标本,分别采用尿沉渣Sternheimer染色法(S染色法)、尿沉渣Sternheimer-Malbin改良染色法(S-M染色法)、Berhe-Muhlberg染色法(B-M染色法)、阿利新兰-中性红染色法(阿-中染色法)及瑞氏-姬姆萨复合染色法(瑞-姬染色法)进行染色,观察5种染色方法最佳效果时的染液与尿液比例及染色时间。结果:尿沉渣S和S-M染色法染液与尿液比例2∶1和1∶1染2~5 min时背景清晰,S染色法有形成分结构清晰易辨别,S-M染色法有形成分结构着色略差,两者比较差别不大(P>0.05);B-M染色法、阿-中染色法及瑞-姬染色法染色法有形成分的着色与背景相似,结构模糊,细菌不着色,杂质较多,差异无统计学意义(P>0.05),但与S、S-M染色方法比较,差异有统计学意义(P<0.05)。结论:尿沉渣S和S-M染色法染色效果佳,尤以S染色法最佳。
Objective: To explore the effects of 5 urinary sediment staining methods. Methods: A total of 180 cases of abnormal urinary sediment samples were stained by Sternheimer staining method( S method),modified Sternheimer-Malbin staining method( S-M method),Berhe-Muhlberg staining method( B-M method),Alcian blue and neutral red staining method( A method) and Wright Giemsa staining method( W method),the dye and urine proportion,the dyeing time were observed when achieved the best effects. Results: S method showed clear background and was easy to identify visible components with the dye and urine proportion of 2 ∶ 1 and 1 ∶ 1,dyeing 2 ~ 5 min,and S-M method dyed slightly worse,the differences between the two methods were not significant( P > 0. 05). Concerning B-M method,A method and W method,the color of visible component was similar to the background,the structure was fuzzy,the bacteria were not colored,and the impurities were more,the differences among the three methods were not significant( P > 0. 05),but compared with S method and S-M method,the differences were significant( P < 0. 05). Conclusion: S method has the best staining effects.

关键词(KeyWords): 尿沉渣;S染色法;S-M染色法;B-M染色法;阿-中染色法;瑞-姬染色法
urinary sediment;S dyeing method;S-M dyeing method;B-M dyeing method;Alcian blue and neutral red staining method;Wright-Giemsa staining method

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作者(Author): 严雪梅;刘宝;夏曙华;
YAN Xuemei;LIU Bao;XIA Shuhua;College of Life Science,Sichuan University;No.1 Affiliated Hospital of Guiyang College of Traditional Chinese Medicine;Departmet of Microbiology and Immunology,Affiliated Hospital of Guizhou Medical University;Institude of Clinical Laboratory,Guizhou Medical University;

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