胡婷;罗鹏;
HU Ting;LUO Peng;The Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education Department of Toxicology,School of Public Health,Guizhou Medical University;

• 1:贵州医科大学公共卫生学院环境污染与疾病监控省部共建教育部重点实验室

摘要(Abstract)：

目的:探讨竹荪多糖对砷中毒大鼠肝损伤的影响。方法:将24只清洁级健康SD大鼠均分为对照组、模型组、竹荪干预组3组,每组雌雄各半;对照组以常规饲料饲养,竹荪干预组和模型组喂饲砷含量50 mg/kg的饲料,竹荪干预组每日以10 g/L的竹荪多糖20 m L/kg灌胃,各组大鼠喂养3个月时取血检测血清谷草转氨酶(AST)及谷丙转氨酶(ALT)活力、总蛋白(TP)及白蛋白(ALB)水平、白蛋白与球蛋白比值(A/G)、粘连蛋白(LN)、透明质酸酶(HA)、Ⅲ型前胶原(PCⅢ)以及Ⅳ型胶原(PCIV)的水平;取大鼠同一肝小叶行HE及Masson染色,观察肝组织病理学改变,并计算肝纤维化增生面积。结果:与对照组相比,竹荪干预组和模型组大鼠血清内AST的活力均升高,ALB水平降低,LN、HA、PCIV水平增高,差异有统计学意义(P<0.05);与模型组相比,竹荪干预组大鼠血清内AST和ALT的活力均降低,TP、A/G、ALB水平升高,LN、PCIV水平降低,差异有统计学意义(P<0.05);HE和Masson染色结果显示,竹荪干预组和模型组均出现不同程度的肝损伤;通过图像分析系统测量,对照组、竹荪干预组、模型组大鼠肝脏增生纤维面积呈增加趋势,竹荪干预组和模型组与对照组相比胶原纤维沉积面积差异均有统计学意义(P<0.05)。结论:竹荪多糖对砷中毒大鼠肝脏有一定的保护作用。
Objective: To investigate the effect of dictyophora polysaccharide on liver injury in arsenic poisoning rats. Methods: A total of 24 healthy adult clean SD rats were randomly divided into control group(normal feed),dictyophora polysaccharide group and exposed group(arsenic content in feed was 50 mg / kg). Dictyophora polysaccharide group received intragastric administration with dictyophora polysaccharide(10 mg / m L) at 20 m L / kg every day. 3 months after feeding,the rats were executed and the blood was collected to detect serum activities of aspartate aminotransferase(AST),alanine aminotransferase(ALT),total protein(TP) and albumin(ALB),albumin to globulin ratio(A / G),liver fibrosis 4 items laminin(LN),hyaluronidase(HA),type III procollagen(PCIII),type IV procollagen(PCIV). HE and Masson staining were performed in the same liver of rats,the pathological changes of liver tissue were observed,and the area of hepatic fibrosis was calculated. Results: Compared with the control group,in dictyophora intervention group and model group rats' serum AST activity were increased,ALB decreased; LN,HA,and PCIV levels increased. The difference was statistically significant(P < 0. 05). Compared with model group,AST and ALT activity in rats' serum of dictyophora polysaccharide intervention group decreased while TP,A / G,ALB,LN,PCIV level decreased,and the difference was statistically significant(P < 0. 05). HE and Masson staining showed that in dictyophora polysaccharide intervention group and model group the liver damage occurred in different degree. Through the image analysis and systematic measure,control group,in dictyophora polysaccharide intervention group and model group the rats' liver fiber hyperplasia area showed an increasing trend,and there was statistically significant difference in collagen deposition area between dictyophora polysaccharide intervention group,model group and control group(P < 0. 05). Conclusion: Dictyophora polysaccharide may has a protective effect on the liver of rats with arsenic poisoning.

关键词(KeyWords)： 竹荪多糖;亚砷酸钠;肝损伤;模型,动物;大鼠,Sprague-Dawley;纤维化
dictyophora polysaccharide;sodium arsenite;liver injury;model,animal;rats Sprague-Dawley;fibrosis

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81160336);; 贵州省科技厅农业攻关计划项目[黔科合NY字(2011)3055号];贵州省科技厅[黔科合J字(2010)2188];; 贵州省教育厅基金项目[黔省专合字(2010)39号]

作者(Author): 胡婷;罗鹏;
HU Ting;LUO Peng;The Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education Department of Toxicology,School of Public Health,Guizhou Medical University;

Email:

DOI: 10.19367/j.cnki.1000-2707.2016.11.005

参考文献(References)：

• [1]Chao D,Fu H,Xu J,et al.Physiochemical and biological properties of phosphorylated polysaccharides from Dictyophora indusiata[J].International Journal of Biological Macromolecules,2014(72):894-899.
• [2]Dong Z,Liu C,Liu Y,et al.Using publicly available data,a physiologically-based pharmacokinetic model and Bayesian simulation to improve arsenic non-cancer dose-response[J].Environment International,2016(92):239-246.
• [3]韩俊洋,吴顺华.地方性砷中毒致肝损伤机制研究进展[J].中国公共卫生,2014(3):374-376.
• [4]吴雪艳,罗鹏,叶建方,等.响应面法优化竹荪菌托多糖的提取工艺[J].南方农业学报,2015(3):480-485.
• [5]郑杨,邹青青,张岱,等.竹荪的化学成分及生理活性研究进展.食品科学技术学报,2013(31):39-45.
• [6]段小明,刘升,贾丽娥,等.竹荪属食用菌国内研究进展[J].食品安全质量检测学报,2015(11):4433-4440.
• [7]周素娟,张晓娜.食用菌保健功能及保健食品应用与开发[J].中国食用菌,2015(1):4-6.
• [8]姚茂琳,张爱华,于春,等.高砷煤烘玉米粉致大鼠砷中毒模型的建立[J].中华预防医学杂志,2013(9):799-805.
• [9]王丹丹.多糖药理作用的研究进展[J].黑龙江科技信息,2015(8):32.
• [10]张海涛,倪娜.砷中毒治疗的研究现状[J].中国地方病学杂志,2011(1):113-114.
• [11]叶敏,文竹,潘文芳,等.红托竹荪多糖抗衰老和降血糖作用研究[J].食品工业科技,2016(7):343-345.
• [12]叶敏,文竹,黄家振,等.红托竹荪多糖抗疲劳和耐缺氧作用研究[J].天然产物研究与开发,2016(3):416-419.
• [13]王宪伟.竹荪多糖的抑菌活性研究[J].安徽农业科学,2013(4):1415.
• [14]Jomova K,Jenisova Z,Feszterovaet M,et al.Arsenic:toxicity,oxidative stress and human disease[J].Journal Of Applied Toxicology,2011(2):95-107.
• [15]邓青芳,周欣,陈华国.多糖抗肝损伤作用及其机制研究进展[J].中国中药杂志,2016(16):2958-2967.

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