贵州医科大学学报

2015, v.40;No.183(12) 1338-1340

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HPLC法同时测定舒肝宁注射液中表告依春和腺苷的含量
Simultaneous Determination of Goitrine and Adenosine in Shuganning Injection by HPLC

何峰;张昀;
HE Feng;ZHANG Yun;School of Pharmacy,Guizhou Medical University;Guizhou Ruihe Pharmaceutical co.,ltd;

摘要(Abstract):

目的:建立HPLC同时测定舒肝宁中表告依春和腺苷含量的方法。方法:色谱柱为Kromasil C18(250 mm×4.6 mm,5μm),柱温30℃,流动相为甲醇和水,梯度洗脱,流速为1.0 m L/min,检测波长245 nm,进样量为5μL,测定舒肝宁中表告依春和腺苷含量。结果:表告依春在1.197~29.92 mg/L(r=1)范围内线性关系良好,平均回收率为99.83%(RSD=1.98%);腺苷在3.808~95.20 mg/L(r=1)范围内线性关系良好,平均回收率为99.19%(RSD=1.87%)。结论:本方法结果准确可靠,重复性好,适用于舒肝宁注射液的质量控制。
Objective: To investigate the method to simultaneously determine goitrine and adenosine in Shuganning injection by HPLC. Methods: The chromatographic column was Kromasil C18( 250 mm× 4. 6 mm,5 μm),column temperature. The mobile phase consisted of methanol and water at a flow rate of 1. 0ml / min. The detection wavelength was at 245 nm and the injection volume was 5 μL. Results: Goitrine showed a good linear relationship within the range of 1. 197 ~ 29. 92 mg / L( r = 1) and the average recovery was 99. 83%( RSD = 1. 98%). Adenosine showed a good linear relationship within the range of 3. 808 ~ 95. 20 mg / L( r = 1) and the average recovery was 99. 19%( RSD = 1. 87%).Conclusion: This method is reliable,simple and suitable for the quality control of Shuganning injection.

关键词(KeyWords): 色谱法,高压液相;舒肝宁注射液;表告依春;腺苷
chromatography,high pressure liquid phase;Shuganning injection;goitrine;adenosine

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技重大专项项目[黔科合重大专项字(2011)6019号]

作者(Author): 何峰;张昀;
HE Feng;ZHANG Yun;School of Pharmacy,Guizhou Medical University;Guizhou Ruihe Pharmaceutical co.,ltd;

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DOI: 10.19367/j.cnki.1000-2707.2015.12.013

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