贵州医科大学学报

1985, (01) 67-69

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

蚤的组织学制片技术
Technique of Serial Section Cutting of Eleas

漆一鸣;
Qi Yiming Department of Biology

摘要(Abstract):

<正> 蚤的组织学研究,是形态学的一部分,对蚤分类学,生理生态,媒介作用以及防制的进一步研究都是重要的。在国外,近三十年来,对蚤内部组织结构的研究进展很快,而在国内迄今仅见对蚤生殖腺发育和成熟的组织学研究(漆一鸣1982,1984a.b)。为了促进国内在这方面的研究,作者特将蚤的组织学制片技术介绍如下: 制作蚤的连续切片和染色的方法,作者主要是参考M.Rothschild(1975)的方法,并作了如下改良:标本包埋前没有使用′Supercedrol′脱水,而是采用酒精逐级上行至苯、蜡内,同时在蜡片下行至水时,所使用的酒精梯度也不相同。在包埋中没有使用′Paraplast′包埋剂和真空包埋,而是根据幼虫、前蛹、蛹和成虫几丁
A technique of serial section cutting at a study on the histological features of fleas is reported in this paper. The technique is a modified Rothschild's(1975)technique. It is recommended on the following: Fixing: The living materials are fixed in Duboscq-Brasil for 12 to 24 hrs at 30 to 35℃. Embedding; The fixed materials are stained in 1%eosin-alcohol for 20 minutes and then transfered into alcohol 95%twice, absolute alcohol twice,equal parts of absolute alcohol and benzene, and benzene,for 1 hr in each. Wax embed in the thermostat for l to 1.5 hrs at 50 to 58°c. Cutting:Sections are usually cut at 6-8μThe sections are transfered to a slide with egg albumen-glycerine and distilled water and spreaded on a warm iron plate, dried in the thermostat for 2 to 8 hrs 40 to 50°c. Staining:(1)了he dried slide is put into xylol for 20 to 30 minutes then transfered into absolute alcohol, 1%colloidin-ether-alcohol, alcoho170%, and distilled water for 3 to 5 minutes in each.(2)Stain in azocarmine for 5 10 minuteS.(3)Wash in distilled water for 1 to 2 minutes.(4)Differeti ate in aniline alcohol。(5)Stop with 1%acetic acid bath for 1 to 2 minutes. (6)Mordantin 5%phosphotungstic acid for 1 hr.(7)Wash rapidly in distilled water。(8)Stain in aniline mixture for 1 hr (9)Put into alcohol 95% twice.absolute alcohol twice,xylol twice for 3 to 5 minutes in each.(10) Mount in Canada balsam.

关键词(KeyWords):

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 漆一鸣;
Qi Yiming Department of Biology

Email:

DOI: 10.19367/j.cnki.1000-2707.1985.01.020

文章评论(Comment):

序号(No.) 时间(Time) 反馈人(User) 邮箱(Email) 标题(Title) 内容(Content)
反馈人(User) 邮箱地址(Email)
反馈标题(Title)
反馈内容(Content)
扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享