贵州医科大学学报

2013, v.38;No.162(03) 253-256

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内毒素致新生SD大鼠肺损伤模型的建立及鉴定
Building and Evaluation of Acute Lung Injury Model with Neonatal Rats Induced by Lipopolysaccharides

贺务实;燕旭东;周克英;
HE Wushi,YAN Xudong,ZHOU Keying(Department of Pediatrics,Shenzhen People's Hospital,The Second Medical College of JI NAN University,Shenzhen 518020,Guangdong,China)

摘要(Abstract):

目的:探讨新生大鼠急性肺损伤模型的建立及其病理特征。方法:采用腹腔注射内毒素(LPS,5 mg/kg)的方法制备新生SD大鼠急性肺损伤动物模型,在光镜下观察肺的病理变化,观察支气管肺泡灌洗液(BALF)中细胞分类情况,用ELISA、RT-PCR法检测血清和肺组织中白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的表达。结果:LPS注射3 h后,光镜下可见明显的肺出血和中性粒细胞浸润;肺湿/干重比(W/D)增加,BALF中多形核白细胞(PMN)数量明显增加,血清中TNF-α和IL-6表达水平和肺组织中TNF-αmRNA水平均显著增高。结论:新生SD大鼠腹腔注射LPS后,能够引起显著的急性肺部炎症反应。
Objective: To explore the establishment and pathological characteristics of acute lung injury model with neonatal rats.Methods: Acute lung injury models of neonatal rats were established via intraperitoneal injection of 5 mg/kg lipopolysaccharides(LPS).The pathological changes were observed with tissue sections with microscopy.The tumor necrosis factor-α(TNF-α) mRNA expression in lung were measured by RT-PCR.The levels of TNF-α and IL-6 were detected with ELISA.Results: Lung hemorrhage accompanied by polymorphonuclear neutrophil infiltration was observed in 3 hours after LPS administration.Wet/dry ratio,and polymorphonuclear leukocytes(PMN) in bronchoalveolar lavage fluid(BALF) increased obviously.Serum TNF-α level and IL-6 level significantly increased.TNF-α mRNA expression in lung also increased.Conclusion: LPS administration to neonatal rats results in acute lung injury accompanied by obvious inflammatory responses.

关键词(KeyWords): 模型,动物内毒素类;炎症;肺损伤;肿瘤坏死因子
model,animal;endotoxins;Inflammation;lung injury;tumor necrosis factor;interleukin-6

Abstract:

Keywords:

基金项目(Foundation): 深圳市2011年科技计划项目(201102147)

作者(Author): 贺务实;燕旭东;周克英;
HE Wushi,YAN Xudong,ZHOU Keying(Department of Pediatrics,Shenzhen People's Hospital,The Second Medical College of JI NAN University,Shenzhen 518020,Guangdong,China)

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DOI: 10.19367/j.cnki.1000-2707.2013.03.010

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