贵州医科大学学报

2008, No.131(02) 129-131

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

蛋白激酶C-γ亚型在慢性染铅小鼠脑皮质中的表达
Expression of PKC-γ Subgroup in Pallium of Mice Treated with Chronic Lead Contamination

高双;闻涛;孙黎光;
GAO Shuang1,WEN Tao2,SUN Liguang3(1.Section of Hygiene and Laboratory Science,School of Public Health,China Medical University,Shenyang 110001,Liaoning,China;2.China Public Health Periodical Office,Shenyang 110005,Liaoning,China;3.Deparment of Biochemistry and Molecular Biology,School of Basic Medicine,China Medical Universit,Shenyang 110001,Liaoning,China)

摘要(Abstract):

目的:探讨铅对小鼠脑皮质中蛋白激酶C-γ亚型(PKC-γ)蛋白表达的影响。方法:小鼠出生第1天起,染铅组母鼠开始通过饮水饲以不同浓度醋酸铅2.4、4.8、9.6mmol/L;幼鼠出生后,先通过哺乳接触铅,断乳后则自行饮用与母鼠相同浓度的含铅水;分别在第14,21,28,35天处死小鼠,用蛋白免疫印记法观察各组小鼠脑皮质区PKC-γ蛋白表达。结果:慢性铅暴露对小鼠脑皮质PKC-γ蛋白表达总体上呈现下降趋势,各染铅组PKC-γ蛋白表达与相应时期对照组相比差异均有统计学意义(P<0.05)。结论:铅扰乱PKC-γ蛋白正常表达。
Objective:To observe the effect of chronic lead contaminant on PKC-γ protein expression in pallium of mice.Methods:Chronic acetic lead contaminant was applied to mice(2.4,4.8,and 9.6 mmol/L),and pups were killed in 14,21,28,35 days respectively.Western blotting was used to observe PKC-γ protein expression in pallium.Results:Compared with that of control group,PKC-γ protein expression of chronic lead exposure mice in all of treated groups was significantly lower(P<0.05).Conclusion:Chronic lead exposure can disturb PKC-γ protein expression in mice.

关键词(KeyWords): 铅中毒,神经系统;蛋白激酶C;蛋白质亚单位;大脑皮质;小鼠
lead poisoning,nervous system;protein kinase C;protein subunits;cerebral cortex;mice

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(39970651)

作者(Author): 高双;闻涛;孙黎光;
GAO Shuang1,WEN Tao2,SUN Liguang3(1.Section of Hygiene and Laboratory Science,School of Public Health,China Medical University,Shenyang 110001,Liaoning,China;2.China Public Health Periodical Office,Shenyang 110005,Liaoning,China;3.Deparment of Biochemistry and Molecular Biology,School of Basic Medicine,China Medical Universit,Shenyang 110001,Liaoning,China)

Email:

DOI: 10.19367/j.cnki.1000-2707.2008.02.006

文章评论(Comment):

序号(No.) 时间(Time) 反馈人(User) 邮箱(Email) 标题(Title) 内容(Content)
反馈人(User) 邮箱地址(Email)
反馈标题(Title)
反馈内容(Content)
扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享