高双;闻涛;孙黎光;
GAO Shuang1,WEN Tao2,SUN Liguang3(1.Section of Hygiene and Laboratory Science,School of Public Health,China Medical University,Shenyang 110001,Liaoning,China;2.China Public Health Periodical Office,Shenyang 110005,Liaoning,China;3.Deparment of Biochemistry and Molecular Biology,School of Basic Medicine,China Medical Universit,Shenyang 110001,Liaoning,China)

• 1:中国医科大学公共卫生学院卫生检验学教研室
• 2:中国公共卫生杂志社
• 3:中国医科大学基础医学院生物化学与分子生物学教研室 辽宁沈阳110001
• 4:辽宁沈阳110005

摘要(Abstract)：

目的:探讨铅对小鼠脑皮质中蛋白激酶C-γ亚型(PKC-γ)蛋白表达的影响。方法:小鼠出生第1天起,染铅组母鼠开始通过饮水饲以不同浓度醋酸铅2.4、4.8、9.6mmol/L;幼鼠出生后,先通过哺乳接触铅,断乳后则自行饮用与母鼠相同浓度的含铅水;分别在第14,21,28,35天处死小鼠,用蛋白免疫印记法观察各组小鼠脑皮质区PKC-γ蛋白表达。结果:慢性铅暴露对小鼠脑皮质PKC-γ蛋白表达总体上呈现下降趋势,各染铅组PKC-γ蛋白表达与相应时期对照组相比差异均有统计学意义(P<0.05)。结论:铅扰乱PKC-γ蛋白正常表达。
Objective:To observe the effect of chronic lead contaminant on PKC-γ protein expression in pallium of mice.Methods:Chronic acetic lead contaminant was applied to mice(2.4,4.8,and 9.6 mmol/L),and pups were killed in 14,21,28,35 days respectively.Western blotting was used to observe PKC-γ protein expression in pallium.Results:Compared with that of control group,PKC-γ protein expression of chronic lead exposure mice in all of treated groups was significantly lower(P<0.05).Conclusion:Chronic lead exposure can disturb PKC-γ protein expression in mice.

关键词(KeyWords)： 铅中毒,神经系统;蛋白激酶C;蛋白质亚单位;大脑皮质;小鼠
lead poisoning,nervous system;protein kinase C;protein subunits;cerebral cortex;mice

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(39970651)

作者(Author): 高双;闻涛;孙黎光;
GAO Shuang1,WEN Tao2,SUN Liguang3(1.Section of Hygiene and Laboratory Science,School of Public Health,China Medical University,Shenyang 110001,Liaoning,China;2.China Public Health Periodical Office,Shenyang 110005,Liaoning,China;3.Deparment of Biochemistry and Molecular Biology,School of Basic Medicine,China Medical Universit,Shenyang 110001,Liaoning,China)

Email:

DOI: 10.19367/j.cnki.1000-2707.2008.02.006

参考文献(References)：

• [1]韩太真,吴馥梅.信息记忆的神经生物学[M].北京:北京医科大学、中国协和医科大学联合出版社,1998:265-266.
• [2]阮迪云.铅影响儿童学习记忆的毒理机制.世界元素医学,1999(3):51-54.
• [3]Long Gj,Rosen JF,Schanne FA.Lead activation of protein kinase C from rat brain.Determination of free calcium,lead,and zinc by19F NMR[J].J Biol Chem,1994(2):834-837.
• [4]Heiman AS,Tonner LE.The acute effect of lead acetate on glucocorticoid regulation of tyrosine aminotransferase in hepatoma cells[J].Toxicology,1995(1～3):57-68.
• [5]Danziger N,Yokoyama M,Jay T,et al.Cellular expres-sion,developmental regulation,and phylogenic conserva-tion of PEA-15,the astrocytic major phosphoprotein and protein kinase C substrate[J].J Neurochem.1995(3):1016-1025.
• [6]邢伟,孙黎光,时利德,等.染铅鼠脑海马PKC活性与LTP的相关性研究[J].卫生毒理学杂志,1996(3):155-156.
• [7]Hussain RJ,Parsons PJ,Carpenter DO.Effects of lead on long-term potentiation in hippocampal CA3vary with age[J].Brain Res Dev Brain Res,2000(2):243-252.
• [8]Chetty CS,Reddy GR,Murthy KS,et al.Perinatal lead exposure alters the expression of neuronal nitric oxide syn-thase in rat brain[J].Int J Toxicol,2001(3):113-120.

文章评论(Comment)：