贵州医科大学学报

2016, v.41;No.192(09) 1064-1067

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NSAIDs肠病大鼠回肠SIgA、IL-1β、IL-8、IL-10水平及树突状细胞数的变化
Changs of SIg A,IL-1β、IL-8、IL-10 Levels and Dendritic Cells in Intestinal Mucosa of Rats with NSAIDs Enteropathy

肖源;车筑平;程华;杨红静;谭庆华;
XIAO Yuan;CHE Zhuping;CHENG Hua;YANG Hongjing;TAN Qinghua;Department of Digestive System,Third Affiliated Hospital of Guizhou Medical University;Digestive Endoscopy Center,Affiliated Hospital of Guizhou Medical University;Department of Clinical Medicine,Guizhou Medical University;Department of Digestive System,West China Hospital University;

摘要(Abstract):

目的:探讨非甾体类消炎药(NSAIDs)肠病时SD大鼠回肠分泌型免疫球蛋白A(SIg A)、白细胞介素-1β(IL-1β)、IL-8、IL-10水平及树突状细胞数的变化。方法:34只清洁级SD大鼠均分为药物损伤模型组(模型组)和对照组,模型组大鼠腹腔注射阿司匹林(100 mg/kg,2次/d),对照组腹腔注射等量生理盐水;造模2周后处死大鼠,在距回盲瓣5 cm近端肠管切取回肠段2 cm,采用ELISA法检测大鼠回肠黏膜组织SIg A、IL-1β、IL-8及IL-10水平,免疫组织化学(IHC)检测回肠黏膜CD205阳性染色的树突状细胞数量。结果:HE染色结果显示,模型组黏膜上皮细胞破坏,黏膜杯状细胞减少,隐窝结构变形,固有腺体排列紊乱,黏膜以及黏膜下层见大量淋巴细胞以及单核细胞;对照组黏膜正常,未被破坏;免疫组织化学染色后,与对照组比较,模型组大鼠CD205阳性细胞增多,IOD值升高(P<0.05);模型组大鼠回肠黏膜SIg A降低(P<0.05),IL-1β、IL-8及IL-10增加,树突状细胞数量增多,差异有统计学意义(P<0.05)。结论:NSAIDs肠病发生后,回肠黏膜保护屏障受损,体液免疫紊乱。
Objective: To investigate the changes of non-steroidal anti-inflammatory drug enteropathy in SD rats with ileal SIg A,interleukin-Iβ,interleukin-8,interleukin-10 and dendritic cells change.Methods: 34 SPF grade Sprague-Dawley rats were randomly divided into 2 groups in equal number.The aspirin group received intraperitoneally injections of 100 mg / kg of aspirin twice a day for 14 days,the control group received equal volume of normal saline. 5 cm from the ileocecal valve in the proximal intestine was cut off by 2 cm after constructing the model for 2 weeks and executed the rats. The levels of SIg A,IL-1β,IL-8 and IL-10 in ileal tissue were detected by ELISA. The quantity of CD205-positive cells in mucosa was tested by immunohistochemistry analysis. Results: Aspirin group compared with control group,the level of SIg A in ileal tissue of aspirin group decreased( P < 0. 05),IL-1β,IL-8,IL-10 and the number of dendritic cells increased( P < 0. 05); mucosa of control group was normal and stayed intact; after IHC staining,CD205 positive cells increased in model rats,comparing with control group,observation showed increased IOD value( P < 0. 05); comparing with control group,SIg A in model group rats ileal mucosa decreased( P < 0. 05),IL-1β,IL-8 and IL-10 increased,dendritic cells increased( P < 0. 05). Conclusion: NSAIDs bowel disease would damage intestinal mucosal barrier and caused thumoral immune disorder.

关键词(KeyWords): 树突状细胞;分泌型免疫球蛋白A;白细胞介素;非甾体类消炎药肠病;大鼠,Sprague-Dawley
dendritic cell;secretory immunoglobulin A;Interleukin;non steroidal anti-inflammatory drug enteropathy;rats;Sprague-Dawley

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基金项目(Foundation): 贵州省科技厅基金(E2011-10)

作者(Author): 肖源;车筑平;程华;杨红静;谭庆华;
XIAO Yuan;CHE Zhuping;CHENG Hua;YANG Hongjing;TAN Qinghua;Department of Digestive System,Third Affiliated Hospital of Guizhou Medical University;Digestive Endoscopy Center,Affiliated Hospital of Guizhou Medical University;Department of Clinical Medicine,Guizhou Medical University;Department of Digestive System,West China Hospital University;

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