黄健;张姝;黄韻祝;韩昉;郑莹莹;江杰;
HUANG Jian,ZHANG Shu,HUANG Yunzhu,HAN Fang,ZHENG Yingying,JIANG Jie (Department of Clinic Biochemistry,Guiyang,Medical College,Guiyang 550004,Guizhou,China)

• 1:贵阳医学院临床生化教研室

摘要(Abstract)：

目的:探讨贵州省汉族人群载脂蛋白M(apoM)基因启动子区域-778位点T→C置换与冠心病的关系。方法:用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)对220例冠心病患者和195例健康对照者的apoM启动子-778位点进行基因筛查。结果:冠心病病例组中apoM启动子基因-778位点C等位基因的频率显著高于健康对照组(19.1%和12.6%,P=0.011)。结论:apoM启动子基因-778位点携带C等位基因可能是冠心病的一个危险的遗传易感因子。
Objective: To investigate the relationship between apolipoprotein M(apoM) gene promoter T-778C polymorhpism and coronary heart disease(CHD) in Han people of Guizhou.Methods: PCR-RFLP was adopted to analyze apoM gene pormoter T-778C polymorphism in 220 CHD patients(CHD group) and 196 healthy people(control group).Results: C allele frequency of apoM T-778 was higher in CHD group(19.1%)than that in control group(12.6%)(P=0.011).Conclusions: C allele at nucleotide-778 of apoM promoter might be a risk factor for genetic susceptibility to CHD.

关键词(KeyWords)： 载脂蛋白类;转录启动子;冠状动脉疾病;心脏病
apolipoproteins;transcription initiation site;coronary disease;heart disease

Abstract:

Keywords:

基金项目(Foundation): 贵州省卫生厅优秀医学青年科技人才专项科研基金(G2008-12);; 大学生创业基金(T2008-15-24)。

作者(Author): 黄健;张姝;黄韻祝;韩昉;郑莹莹;江杰;
HUANG Jian,ZHANG Shu,HUANG Yunzhu,HAN Fang,ZHENG Yingying,JIANG Jie (Department of Clinic Biochemistry,Guiyang,Medical College,Guiyang 550004,Guizhou,China)

Email:

DOI:

参考文献(References)：

• [1]Xu N,Dahlback B.A novel human apolipoprotein(apoM)[J].J BiolChem,1999(44):31286-31290.
• [2]Ni N,Zhu X,Liu Y,et al.Single nucleotide polymor-phisms in the proximal promoter region of apolipoprotein-Mgene(apoM)confer the susceptibility to development oftype 2 diabetes in Han Chinese[J].Diabetes Metab ResRev 2007(23):21-25.
• [3]Wolfrum C,PoyMN,StoffelM.Apolipoprotein M is re-quired for preβ-HDL formation and cholesterol efflux toHDL and protects against atherosclerosis[J].Nat Med,2005(4):418-422.
• [4]Hhi DY,Howles PN.Carboxyl ester lipase:structure-function relationship and physiological role in lipoproteinmetabolism and atherosclerosis[J].Journal of lipid re-search,2002(43):2017-2030.
• [5]Dahlback B,Nielsen LB.ApolipoproteinM-a nover play-er in high-density lipoprotein metabolism and atheroscle-rosis.CurrOp in Lipidol,2006(17):291-295.
• [6]Christoffersen C,Nielsen LB,Olof Axler,et al.Isolationand characterization of human apolipoprotein M-containinglipoproteins[J].Journal of Lipid Research,2006(47):1833-1843.

文章评论(Comment)：