贵州医科大学学报

2007, No.124(01) 37-41

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葛根素预处理保护心肌缺血再灌注损伤的机制
The Mechanism of Protective Effect of Puerarin on Myocardial Ischemic Reperfusion Injury in Rats

高翔;高全;李涛;陆德琴;
GAO Xiang,GAO Quan,LI Tao,LU Deqin(1.Department of Pathophysiology,Guiyang Medical College,Guiyang 550004;2.Staff and Workers' Hospital Attached to Guiyang Xin Tian Photoelectric Science and Technology Limited Company,Guiyang 550018)

摘要(Abstract):

目的:探讨线粒体ATP敏感性钾通道(mitoKATP)是否参与葛根素(puerarin,PUE)预处理保护心肌缺血再灌注损伤的机制。方法:开胸结扎左冠状动脉前降支(LAD),复制大鼠心肌缺血再灌注损伤模型,设立假手术(sham)组、缺血再灌注(I/R)组、PUE预处理组,PUE预处理加mitoKATP特异性阻断剂5-hydroxydecanoate(5-HD)组和5-HD组,采用TTC法测定心肌梗塞面积,TUNEL法检测心肌细胞凋亡情况,并测定血清肌酸激酶(CK)活力。结果:I/R组较sham组心肌梗塞面积、血清CK活力以及心肌细胞凋亡指数显著增高;与I/R组比较,PUE预处理组心肌梗塞面积明显缩小,血清CK活力和心肌细胞凋亡指数显著降低;PUE加5-HD组与PUE预处理组相比心肌梗塞面积增大,心肌细胞凋亡增加,血清CK活力增高;5-HD组与I/R组相比以上指标差异均无显著意义。结论:葛根素预处理明显减轻了大鼠心肌I/R损伤,其心肌保护效应可能部分通过开放mitoKATP通道的途径产生。
Objective:To investigate the role of mitochondrial KATP channel(mitoKATP) in the protective effect of puerarin(PUE) on myocardial ischemia-reperfusion injury in rats.Methods: Rat heart ischemia-reperfusion injury(I/R) models were induced by occluding the left anterior descending coronary artery for 45 min and reperfusion for 120 min.Fifty-five rats were randomly divided into five groups(11 in each):(1) Group sham(did not receive operation);(2) group I/R;(3) group PUE(received PUE at a dose of 100 mg/kg intravenously 5 min before ischemia);(4) group P5D(received 5-hydroxydecanoate,a mitoKATP specific inhibitor,at a dose of 5 mg/kg intravenously 15 min before ischemia and PUE treatment at 5 min before ischemia);and(5) group 5-HD(received 5-hydroxydecanoate alone 15 minutes before ischemia).TTC dyeing was used to determine the myocardial infarction size.Cardiac myocytes apoptosis was detected by using TUNEL.Creatine kinase activity(CK) in serum was measured simultaneously.Results: Infarct size,cardiomyocytes apoptosis and CK activity in serum of group I/R were obviously higher than those in group sham(P<0.05 or P<0.01).Compared with group I/R,all these parameters in PUE groups were significantly lower(P<0.05 or P<0.01).While,in group P5D,all these parameters were significantly higher than those in group PUE.There was no significant difference found among the parameters of groups P5D,I/R and 5-HD.Conclusions: Preconditioning with puerarin can significantly reduce myocardial ischemia-reperfusion injury in rats.The protective effect of puerarin to myocardium is partly via opening the mitoKATP channel.

关键词(KeyWords): 心肌再灌注损伤;心肌缺血;心肌梗塞;大鼠,Wistar;葛根素;线粒体ATP敏感性钾通道
myocardial reperfusion injury;myocardial ischemia;myocardial infarction;rats, Wistar;puerarin;mitochondrial KATP channel

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作者(Author): 高翔;高全;李涛;陆德琴;
GAO Xiang,GAO Quan,LI Tao,LU Deqin(1.Department of Pathophysiology,Guiyang Medical College,Guiyang 550004;2.Staff and Workers' Hospital Attached to Guiyang Xin Tian Photoelectric Science and Technology Limited Company,Guiyang 550018)

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DOI: 10.19367/j.cnki.1000-2707.2007.01.012

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