鼠肝细胞内GSH、GPx和Ca~(2-)、~2Mg-ATPase的含量改变与增龄Chanses of GSH, GPx and Ca~2. Mg~2 -ATPase in the Rat Liver with Aging
卢步峰;唐筑灵;张小蕾;
Lu Bufeng, et al Zhenjiang Medical College
摘要(Abstract)：
本文观察了不同年龄组大鼠肝细胞膜中钙镁-腺苷三磷酸酶(Ca~(2+),Mg~(2+)-ATPase)的活性,同时测定胞液中还原型谷胱甘肽(GSH)和谷胱甘肽过氧化物酶(GPx)的含量。结果显示,上述三种物质随增龄其含量改变的趋势相同。它们在中龄组的水平均显著高于青龄组(P<0.05或P<0.01);与中龄组相比,在高龄组其水平均显著下降(P<0.05或P<0.01);除Ca~(2+),Mg~(2+)-ATPase活力稍低于青龄组外,高龄组GSH和GPx的水平仍高于青龄组(P<0.05或P<0.01)。提示上述三种物质含量的变化均可作为研究细胞衰老的客观指标。
The levels of the membrane Ca~2, Mg~2-Adenosine Triphosphatase (Ca~2, Mg~(2+)-ATPase) and the cytoplasm reduced Glutathione (GSH) and Gluthione Peroxidase (GPx) in the rat livers were studied in various age groups. The results showed that. 1. The levels of GSH and GPx in the rat liver exhibited a same tendency with aging:the mid-aged aroup (12 months) significantly higher than the yonng group (3 months) (P<0.01) ; the high-aged group (18 months) lower than the mid-aged (P<0.05 or P<0.01), but still higher than the young group (P<0.05 or P<0.01). 2.The activity of Ca~2, Mg~(2+)-ATPase:the mid-aged group significantly higher than the young group(P<0.05); the high-aged group lower than the mid-aged group(P<0.05). The results suggest that the contents of GSH, GPx and Ca~(2+), Mg~(2+)-ATPase in the rat liver are age-related. It is concluded that all above parameters may be used as indications of aging.
关键词(KeyWords)：
谷胱甘肽还原酶类;腺苷三磷酸酶类;钙;镁;谷胱甘肽过氧化酶类;肝;大鼠
glutathione reductase;adenosine triphosphatase;calcium;magnesium;glutathione peroxidase;liver;rats
基金项目(Foundation):
作者(Author):
卢步峰;唐筑灵;张小蕾;
Lu Bufeng, et al Zhenjiang Medical College
Email:
DOI: 10.19367/j.cnki.1000-2707.1993.01.008
参考文献(References)：
- 1 Harfeman DG, et al. Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rat. J Nutrition, 1974, 104 (5) : 580
- 2 Lenzetta PA,et al. An improved assay for nanomole amounts of inorganic phosphate. Anal Biochem, 1979, 100 (1) : 95
- 3 Chen TS, et al. Life span profiles of glutathione and acetaminophen detoxification. Drug Metab Dispos, 1990, 18 (6) : 882
- 4 Perrin R, et al. Dlood activity of Cu/Zn superoxide dismutase, glutathione peroxidase and catalase in Alzheimer's disease: a case-control study. Gerontology, 1990, 36 ( 5-6) : 306
- 5 Jain A, et al, Glutathione deficincy lead to mitochonrial damage in brain, Proc Natl Acad Sci USA, 1991, 88 (5) : 1913
- 6 Pall ardo FV, et al. Physiological changes in glutathione metabolism in foetal and newborn rat liver. Biochem J, 1991, 274 (3) : 891
- 7 Viani P, et al. Age-related differences in synaptosomal peroxidative damage and membrane properties. J Neurochem, 1991, 56(1) : 253
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