刘帮慧;
LIU Banghui;Jingzhou Central Hospital of Hubei Province;

• 1:荆州市中心医院

摘要(Abstract)：

目的:探讨地黄饮子对急性脑缺血大鼠的学习记忆能力影响。方法:选取Wistar大鼠72只随机均分为实验1组、实验2组、模型组和正常对照组,前3组大鼠复制急性脑缺血模型,正常对照组大鼠手术但不给予脑缺血;实验1组造模后每日灌服地黄饮子汤36 g/kg,实验2组造模后每日灌服尼莫地平12.5 mg/kg,模型组和正常对照组造模后1次/d盐水灌胃,各组大鼠连续灌胃3周;所有大鼠进行Morris水迷宫定位航行实验记录逃避潜伏期测试学习能力,跳台实验记录逃避潜伏期评价记忆能力,并用电镜图像分析系统计算4组大鼠脑梗死面积。结果:与正常对照组比较,模型组大鼠定位航行实验潜伏期明显延长,差异有统计学意义(P<0.05);与模型组比较,实验1组和实验2组大鼠的定位航行实验潜伏期明显缩短,差异有统计学意义(P<0.05);与正常对照组比较,模型组大鼠跳台实验潜伏期明显延长,差异有统计学意义(P<0.05);与模型组比较,实验1组和实验2组大鼠的跳台实验潜伏期明显缩短,差异有统计学意义(P<0.05);实验1组脑梗死面积明显小于模型组和实验2组,差异有统计学意义(P<0.05)。结论:地黄饮子可以提高脑缺血再灌注大鼠的学习能力和记忆能力,减少缺血对脑组织神经元的损伤。
Objective: To explore the effect of Rehmannia Yin Zi on learning and memory ability of acute cerebral ischemia rats. Methods: 72 Wistar rats were randomly divided into experimental group1,experimental group 2,model group and normal control group. The acute cerebral ischemia model was established in the first 3 groups of rats while the normal control group did not received cerebral ischemia surgery. Experiment 1group received daily intragastric administration of Dihuangyinzi Decoction(36 g /kg) after modeling,experimental group 2 received daily intragastric administration of nimodipine(12. 5 mg / kg) after modeling,and model group and normal control group received 1 / d saline after modeling. All rats were administered orally for 3 consecutive weeks. All rats were subjected to Morris water maze navigation experiment to for memory capacity evaluation. Electron microscope image analysis system was adopted to calculate cerebral infarction area for the 4 groups of rats. Results: Compared with normal control group,the experimental latency of model group was significantly prolonged,and the difference was statistically significant(P < 0. 05). Compared with model group,the experimental latency in experimental group 1 and experimental 2 were significantly shortened,the difference was statistically significant(P < 0. 05). Compared with normal control group,the step- down test latency of rats in model group was significantly increased,and the difference was statistically significant(P <0. 05). Compared with model group,the step-down test latency of experimental group 1 and experimental group 2 rats were significantly shorter,and the difference was statistically significant( P <0. 05); The cerebral infarction area in experimental group1 was significantly smaller than the that of model group and experimental group 2,and the difference was statistically significant( P < 0. 05).Conclusion: Rehmannia Yin Zi can improve learning and memory ability of cerebral ischemia reperfusion rats,and reduce the damage of brain neurons resulted from ischemia.

关键词(KeyWords)： 脑缺血再灌注;大鼠;学习记忆能力
cerebral ischemia reperfusion;rats;learning and memory ability

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 刘帮慧;
LIU Banghui;Jingzhou Central Hospital of Hubei Province;

Email:

DOI: 10.19367/j.cnki.1000-2707.2016.11.013

参考文献(References)：

• [1]Go AS,Mozaffarian D,Roger VL,et al.Heart disease and stroke statistics-2013 update:a report from the American Heart Association[J].Circulation,2013(1):143.
• [2]Liu L,Wang D,Wong KS,et al.Stroke and stroke care in China:huge burden,significant workload,and a national priority[J].Stroke,2011(42):3651.
• [3]张文艳,孙宝飞,余资江,等.原花青素对小鼠脑缺血再灌注损伤的神经保护机制[J].贵阳医学院学报,2015(4):352-355.
• [4]邹伟,于婷婷,王冬,等.头针透穴法对脑出血大鼠脑组织中β-cate-nin表达影响的实验研究[J].中医药信息,2014(1):76-79.
• [5]谢宁,刘艳丽,宋琳,等.地黄饮子的实验研究进展[J].中华中医药学刊,2015(12):2823-2325.
• [6]谢宁,刘艳丽,宋琳,等.地黄饮子的实验研究进展[J].中华中医药学刊,2015(12):2823-2325.
• [7]周妍妍,姚辛敏,何秀丽,等.地黄饮子对老年性痴呆神经保护作用的实验研究[J].中医药学报,2011(2):58-61.
• [8]周妍妍,姚辛敏,何秀丽,等.地黄饮子对Aβ诱导的PC12细胞SOD mRNA表达影响的研究[J].中西医结合心脑血管病杂志,2011(6):704-706.
• [9]谢宁,张杨,宋琳,等.地黄饮子对快速老化小鼠SAMP8定位导航学习记忆影响的研究[J].中华中医药学刊,2010(7):1357-1358.
• [10]马涛,闫妍,张允岭,等.补肾填精法对阿尔茨海默病小鼠PI3K/Akt通路激活及氧化应激的影响[J].北京中医药,2014(7):492-495.
• [11]谢宁,于淼,姚辛敏.含地黄饮子脑脊液对PC-12细胞氧化应激的影响[J].时珍国医国药,2012(8):1885-1886.
• [12]李子军,刘春娜.地黄饮子对海马神经元缺氧损伤的保护机制[J].中成药,2012(8):1421-1424.
• [13]张赓,吴金娟,姜淼,等.中医药治疗阿尔兹海默病的研究进展[J].中国实验方剂学杂志,2014(6):217-222.

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