贵州医科大学学报

2012, v.37;No.158(05) 465-468

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不同温度及pH值对人结肠腺癌细胞模型的影响
Effects of Different Temperature and PH Value on Caco-2 Cell Model

刘影;李月婷;查雨锋;王静娴;肖婷婷;李靖;董莉;傅晓钟;黄勇;王永林;
LIU Ying,LI Yueting,ZHA Yufeng,WANG Jingxian,XIAO Tingting,LI Jing, DONG Li,FU Xiaozhong,HUANG Yong,WANG Yonglin(School of Pharmacology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

摘要(Abstract):

目的:建立人结肠腺癌细胞系(Caco-2)细胞模型并对其药物研究的方法进行初步探讨。方法:通过测定跨细胞膜电阻(TEER)、荧光素通透量及碱性磷酸酶(AKP)评价Caco-2细胞模型,用MTT法考察HBSS缓冲液在不同pH值、不同温度及不同时间条件下对Caco-2细胞的毒性。结果:各项指标的测定值符合要求,表明本实验建立的Caco-2细胞模型的完整性、紧密性和通透性良好;进行药物Caco-2细胞吸收潜性研究时,药物作用4 h之内对Caco-2细胞模型没有影响。结论:建立的Caco-2细胞模型可用于药物吸收机制研究。
Objective: To establish the Caco-2 cell model,and to explore the method for applying it in drug research.Methods: Transepithelial electrical resistance(TEER),the permeability of fluorescein and the expression of alkaline phosphatase(APK) were used to evaluate Caco-2 cell model.MTT method was used to detect the toxicity of HBSS buffer to Caco-2 cell on the condition of different pH,different temperature and cultivation time.Results: Test results of all the indexes met the requirements,which indicated that integrity,consistency and permeability of the established Caco-2 cell model were good.The model was not affected when Caco-2 cell was used in drug absorption study within 4 hours.Conclusions: The established Caco-2 cell model can be used to study the absorption mechanism of drugs.

关键词(KeyWords): 结肠;腺癌;细胞,培养的;碱性磷酸酶;毒性试验
colon;adenocarcinoma;cells,cultured;alkaline phosphatase;toxicity tests

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(No.20962004,No.81060359);; 贵州省中药现代化科技产业研究开发专项[黔科合设字(2010)5019];; 贵州省社会发展攻关计划[黔科合SY(2010)3046号];; 贵州省科技厅攻关计划[黔科合GY字(2008)3028]

作者(Author): 刘影;李月婷;查雨锋;王静娴;肖婷婷;李靖;董莉;傅晓钟;黄勇;王永林;
LIU Ying,LI Yueting,ZHA Yufeng,WANG Jingxian,XIAO Tingting,LI Jing, DONG Li,FU Xiaozhong,HUANG Yong,WANG Yonglin(School of Pharmacology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

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DOI: 10.19367/j.cnki.1000-2707.2012.05.003

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