贵州医科大学学报

2016, v.41;No.190(07) 750-754

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新疾病模式生物斑马鱼G6PD酶活性的动态研究
Dynamic Research G6PD Enzyme Activity of New Disease Patterns Zebrafish

宋锦;李莉;尚鲁俊;吴西军;周艳华;何志旭;舒莉萍;
SONG Jin;LI Li;SHANG Lujun;WU Xijun;ZHOU Yanhua;HE Zhixu;SHU Liping;Department of Immunology,Guizhou Medical University;The Center of Stem Cell and Tissue Engineering Research,Guizhou Medical University;Department of Paediatrics,Guizhou Medical University;Experimental Animal Center,Guizhou Medical University,Guizhou Medical University;

摘要(Abstract):

目的:观察不同发育时期的斑马鱼胚胎、成年斑马鱼不同组织的G6PD酶活性及g6pd mRNA的表达。方法:收集受精后24 h(24 hpf)、48 hpf、受精后5 d(5 dpf)及15 dpf的野生型斑马鱼胚胎,同时收集成年斑马鱼的脑、肌肉、鳃、皮肤、眼睛及肠等组织,运用改良G6PD定量比值法和RT-PCR法检测上述胚胎或组织的G6PD酶活性及g6pd mRNA表达水平。结果:G6PD酶活性、g6pd mRNA表达水平在24 hpf、48 hpf、5 dpf及15 dpf比较,差异无统计学意义(P>0.05);在成年斑马鱼的脑、肌肉、鳃、皮肤、眼睛及肠等不同部位组织比较,差异也无统计学意义(P>0.05)。结论:野生斑马鱼G6PD酶活性、g6pd mRNA表达水平不受胚胎发育时期和组织部位的影响。
Objective: To observe G6 PD enzyme activity and G6 PD mRNA expression in different tissues of zebrafish embryos and adult zebrafish at different developmental stages. Method: After fertilization,24 hpf( 24 hour post-fertilization),48 hpf,5 dpf( 5 day post-fertilization) and 15 dpf wild type zebrafish embryos were collected,and the brain,muscle,gill,skin,eyes and intestine of adult zebrafish were collected simultaneously. The modified G6 PD quantitative ratio assay and RT-PCR were adopted to detect G6 PD activity and mRNA expression levels in zebrafish embryos and tissues. Result:There was no statistically significant difference in G6 PD activity and mRNA expression levels between24 hpf,48 hpf,5 dpf and 15 dpf( P > 0. 05). There was also no statistically significant difference in G6 PD activity and mRNA expression levels between brain,muscle,gill,skin,eyes and intestine( P >0. 05). Conclusion: The G6 PD activity and mRNA expression level of wild zebrafish is not affected by embryonic development period and tissue site.

关键词(KeyWords): 斑马鱼;胚胎;葡萄糖6磷酸脱氢酶;酶活性;mRNA
zebrafish;embryo;glucose-6-phosphate dehydrogenase;enzymatic activity;mRNA

Abstract:

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基金项目(Foundation): 国家自然科学基金项目资助(31360285)

作者(Author): 宋锦;李莉;尚鲁俊;吴西军;周艳华;何志旭;舒莉萍;
SONG Jin;LI Li;SHANG Lujun;WU Xijun;ZHOU Yanhua;HE Zhixu;SHU Liping;Department of Immunology,Guizhou Medical University;The Center of Stem Cell and Tissue Engineering Research,Guizhou Medical University;Department of Paediatrics,Guizhou Medical University;Experimental Animal Center,Guizhou Medical University,Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2016.07.002

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