贵州医科大学学报

2013, v.38;No.164(05) 458-460

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海洛因依赖大鼠中脑腹侧被盖区β-EP的表达
Expression of β-EP in Brain Ventral Tegmental Area of Heroin Dependent Rats

李一欣;梁文妹;
LI Yixin;LIANG Wenmei;Department of Histology and Embryology,Guiyang Medical College;

摘要(Abstract):

目的:探讨海洛因依赖对大鼠中脑腹侧被盖区(VTA)β-内啡肽(β-endrophin,β-EP)表达的影响。方法:成年雄性SD大鼠55只,分为海洛因依赖组(HDG)及生理盐水对照组(SCG)各25只,正常对照组(NCG)5只,建立海洛因依赖模型;分别于第10、17、24、31、38天取脑组织,用免疫组织化学SABC法显示VTA区β?EP阳性细胞,并用图像分析法测定平均光密度值。结果:显微镜观察显示,与盐水对照组和正常对照组比较,海洛因依赖组VTA区的β?EP阳性细胞免疫反应强度减弱,染色变浅;图像分析测定大鼠VTA区β?EP阳性细胞的平均光密度值,盐水对照组与正常对照组比较差异无统计学意义(P﹥0.05),海洛因依赖组与正常对照组和盐水对照组比较均显著降低(P﹤0.05),且随着依赖时间延长呈下降趋势。结论:在海洛因依赖期间,VTA区β?EP表达减少,提示海洛因抑制了内源性阿片肽的产生。
Objective: To investigate the effect of heroin dependence on expression of β-endrophin(β-EP) in ventral tegmental area(VTA) of rat brain.Methods: Fifty-five male adult SD rats were randomly divided into a normal control group(group NC,n = 5),a saline control group(group SC,n = 25) and a heroin dependent group(group HD,n = 25).Heroin-dependent rat models were set up by subcutaneous injection of heroin and the brain tissue were excised on the 10th,17th,24th,31st,and 38thdays.Immunohistochemical SABC method was used to detect the β-EP positive cells in VTA,and average optical density was examined by using imagine analysis method.Results: The immune reaction intensity in β-EP positive cells of group HD was weaker,and the color was lighter than those of groups NC and SC(P ﹤ 0.05).The average optical density of β-EP positive cells was not significantly different between groups SC and NC,but it's lower in group HD than in the 2 groups,and showed a downtrend along with time(P ﹤ 0.05).Conclusions: The expression of β-EP in VTA decreases during heroin dependence,which suggests that heroin inhibits the production of endogenous opioid.

关键词(KeyWords): 海洛因依赖;中脑被盖;β内啡肽;免疫组织化学;大鼠,Sprague-Dawley
heroin dependence;tegmentum mesencephali;beta-endorphin;immunohistochemistry;rats,Sprague-Dawley

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(81060110);; 贵州省优秀科技教育人才省长专项资金[黔省专合字201148号]

作者(Author): 李一欣;梁文妹;
LI Yixin;LIANG Wenmei;Department of Histology and Embryology,Guiyang Medical College;

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DOI: 10.19367/j.cnki.1000-2707.2013.05.004

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