贵州医科大学学报

2018, v.43;No.214(07) 794-797

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膝关节炎家兔外周血BTLA、IL-1、TNF-α、IL-10及部分血小板参数的变化
Changes of BTLA,IL-1,TNF-α,IL-10,and Some Thrombocyte Parameters in Peripheral Blood of Knee Osteoarthritis Model Rabbits

张可训;张建鹏;兰亚娟;
ZHANG Kexun;ZHANG Jianpeng;LAN Yajuan;Sanyuan County Hospital;

摘要(Abstract):

目的:探讨B、T淋巴细胞衰减因子(BTLA)、血清白介素-1(IL-1)、肿瘤坏死因子-α(TNF-α)、白介素-10(IL-10)及部分血小板指标在膝关节炎(OA)新西兰家兔外周血中的变化。方法:30只新西兰家兔均分为模型组、治疗组和假手术组,切断模型组和治疗组新西兰家兔前交叉韧带、剪除内侧半月板、建立兔膝OA模型,假手术组仅打开关节腔;手术结束3周时流式细胞技术检测3组大鼠外周血CD3~+BTLA~+T细胞、CD4~+BTLA~+T细胞、CD8~+BTLA~+T细胞及调节性T细胞(Treg),ELISA法检测血清IL-1、TNF-α、IL-10水平,全自动生化分析仪检测血小板计数(PLT)、血小板压积(PCT)、血小板分布宽度(PDW)及血小板平均体积(MPG)。结果:造模成功后3周时模型组家兔外周血IL-1、TNF-α、IL-10水平高于另外两组(P<0.05),CD3~+BTLA~+T细胞、CD4~+BTLA~+T细胞、CD8~+BTLA~+T细胞及Treg百分比均明显低于另外两组(P<0.05),PLT及PCT均明显低于另外两组(P<0.05);模型组家兔外周血PLT与CD3~+BTLA~+T细胞、CD4~+BTLA~+T细胞及Treg水平成正相关;PCT、PDW与CD8~+BTLA~+T细胞水平成正相关,MPV与CD4~+BTLA~+T细胞水平成正相关(P<0.05)。结论:BTLA阳性T淋巴细胞水平、IL-1、TNF-α、IL-10以及部分血小板参数可能参与了OA的发生发展。
Objective: To explore the changes of B-lymphocyte and T-lymphocyte attenuation factors( BTLA),serum interleukin-1( IL-1),tumor necrosis factor-α( TNF-α),and interleukin-10( IL-10)and some thrombocyte parameters in New zealand rabbit knee osteoarthritis( OA) model. Methods:Thirty New Zealand white rabbits were randomly divided into three groups: sham operation( group S),treatment group( group T),and model group( group M). Rabbit knee OA model was established by cut anterior cruciate ligament and medial meniscus in groups T and M,while rabbits in group S only received operation of opening knee articular cavity. In 3 months after the operations,CD3~+BTLA~+T cells,CD4~+BTLA~+T cells,CD8~+BTLA~+T cells and Treg cells were detected by flow cytometry,levels of IL-1,TNF-α and IL-10 were measured by enzyme-linked immunosorbent assay( ELISA),and platelet count( PLT),platelet cubic measure( PCT),platelet distribution width( PDW),and platelet mean volume( MPV) were measured by automatic biochemical analyzer. Results: In 3 weeks after the model was successfully established,the levels of IL-1,TNF-α,and IL-10 in group M were higher than those in groups S and T( P < 0. 05),while cell counts of CD3~+BTLA~+T,CD4~+BTLA~+T,CD8~+BTLA~+T,and Treg percentage in group M were significantly lower than those in groups S and T( P <0. 05). PLT and PCT of group M were lower than those of groups S and T( P < 0. 05). In peripheral blood of rabbits in group M,PLT was positively correlated with CD3~+BTLA~+T cells,CD4~+BTLA~+T cells and Treg level( P < 0. 05),PCT and PDW were positively correlated with CD8~+BTLA~+T cells( P < 0. 05),and MPV was positively correlated with CD4~+BTLA~+T cells( P < 0. 05). Conclusion:BTLA,T-lymphocyte levels,IL-1,TNF-α,IL-10 and some platelet parameters might participate the occur and development of OA.

关键词(KeyWords): 骨关节炎;B、T淋巴细胞衰减因子;白介素-1;肿瘤坏死因子-α;白介素-10;血小板;兔
osteoarthritis;B,T-lymphocyte attenuation factors;interleukin-1;tumor necrosis factor-α;interleukin-10;platelets;rabbits

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作者(Author): 张可训;张建鹏;兰亚娟;
ZHANG Kexun;ZHANG Jianpeng;LAN Yajuan;Sanyuan County Hospital;

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DOI: 10.19367/j.cnki.1000-2707.2018.07.011

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