贵州医科大学学报

2010, (01) 4-7+12

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天麻微卫星DNA分子标记与天麻素含量的关系
A Primary Study on the Relationship between Microsatellite DNA of Gastrodia elata Blume and Gastrodin Content

郑伟耀;宋聚先;王晓丽;李宝忠;何国栋;
ZHENG Weiyao1,SONG Juxian1,WANG Xiaoli1,LI Baozhong2,HE Guodong1(1.Department of Medical Plant and Pharmacognosy,School of Pharmacology,Guiyang Medical College,Guiyang 550004,Guizhou,China;2.Guizhou Jiacheng Pharmaceuticals Company,Zunyi 563000,Guizhou,China)

摘要(Abstract):

目的:探讨天麻遗传变异与其品质的关系,为优良种质的选育提供科学途径。方法:采用CTAB法提取天麻DNA,进行PCR扩增,用微卫星DNA分子技术(SSR)标记DNA;用高效液相色谱法检测不同种质天麻的天麻素含量,分析两者之间的关系。结果:出现A条带类型的样品天麻素含量较高,7EKYC、9EDF;24GBJ、22GZJ;1FDJ、2FLL;4VDF、6EKYW;18GWAC、8EWD,分别聚到一起,遗传距离较小。结论:特异引物扩增位点与天麻素含量有关,高天麻素含量与SSR高分子量条带有关,其关系有待进一步研究。
Objective:To investigate the associativity between the genetics and variation of Gastrodia elata Blume and its quality,so as to provide scientific path for good species selection.Methods:DNA of Gastrodia elata Blume was extracted by CTAB method,amplified by PCR and marked by SSR technique;Gastrodin content was detected by HPLC.The relation between SSR fingerprinting and Gastrodin contents was analyzed.Results:Gastrodin contents were relatively high in specimens with type A bands.Genetic distances between 7EKYC and 9EDF,24GBJ and 22GZJ,1FDJ and 2FLL,4VDF and 6EKYW,18GWAC and 8EWD were small.Conclusions:The sites for special primer amplification relate to Gastrodin content,and Gastrodin content relates to SSR fingerprinting,which needs further research.

关键词(KeyWords): 天麻;天麻素;生物学标记;SSR分子标记
Gastrodia elata;Gastrodin;biological marker;SSR molecule marker

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基金项目(Foundation): 科技部2004BA721A33;; 贵州省中药现代化科技产业:黔科合中药专字[2003]51号、黔科合农社字(2001)1125号

作者(Author): 郑伟耀;宋聚先;王晓丽;李宝忠;何国栋;
ZHENG Weiyao1,SONG Juxian1,WANG Xiaoli1,LI Baozhong2,HE Guodong1(1.Department of Medical Plant and Pharmacognosy,School of Pharmacology,Guiyang Medical College,Guiyang 550004,Guizhou,China;2.Guizhou Jiacheng Pharmaceuticals Company,Zunyi 563000,Guizhou,China)

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