贵州医科大学学报

2018, v.43;No.215(08) 874-878

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芍药苷对人胰腺癌Panc-1细胞系增殖和凋亡的影响
Effects of Paeoniflorin on Proliferation and Apoptosis of Pancreatic Cancer PANC-1 Cell Line

黄巍;孙诚谊;喻超;潘耀振;田舍;张乙凡;陈世裕;江建新;
HUANG Wei;SUN Chengyi;YU Chao;PAN Yaozhen;TIAN She;ZHANG Yifan;CHEN Shiyu;JIANG Jianxin;Guizhou Medical University;Department of Hepatobiliary Surgery,the Affiliated Hospital of Guizhou Medical University;Renmin Hospital of Wuhan University;Hubei Provincial Institute of Digestive Diseases;

摘要(Abstract):

目的:探究芍药苷(PF)对人胰腺癌Panc-1细胞增殖及凋亡的影响。方法:将人胰腺癌Panc-1细胞分为对照组(0μmol/L PF)和12.5、50、200及800μmol/L PF组,用细胞增殖实验(CCK8)和平板克隆实验检测PF对人胰腺癌Panc-1细胞增殖的影响,用Annexin V-FITC/PI双染试剂染色实验检测人胰腺癌Panc-1细胞凋亡,免疫印迹实验检测PF对人胰腺癌Panc-1细胞Caspase-3及Bcl-2蛋白表达水平的影响。结果:CCK8实验显示Panc-1细胞增殖能力随药物浓度的增加而减少(P<0.05),平板克隆实验显示细胞集落数量随药物浓度的增加而减少(P<0.05),Annexin V-FITC/PI双染试剂染色实验显示细胞凋亡发生率随药物浓度的增加而增加(P<0.05);Western blot实验结果显示Caspase-3、Cleave caspase3的表达量随PF浓度的增加而增加(P<0.05),Bcl-2与其相反。结论:PF可以抑制人胰腺癌Panc-1细胞的增殖、促进其凋亡,其机制可能是激活了Caspase途径。
Objective: To investigate the effect of Paeoniflorin( PF) on the proliferation and apoptosis of pancreatic cancer Panc-1 cells. Methods: Pancreatic cancer Panc-1 cells were divided into 0,12. 5,50,200 and 800 μmol/L PF group; The effect of different concentrations of Paeoniflorin on the proliferation of Panc-1 by CCK-8 assay and Plate cloning. Apoptosis of Panc-1 was detected by Annexin V-FITC/PI double staining test and Western blot was adopted to detect effect of PF on Panc-1 Caspase-3 and Bcl-2 protein expression. Results: CCK8 experiments indicated that proliferation of Panc-1 reduced with the increase of concentration level of PF( P < 0. 05); plate cloning experiment showed that colony formation number reduced with the increase of concentration level of PF( P <0. 05); Annexin V-FITC/PI double staining test indicated that apoptosis occurrence rate increased with the increase of concentration level of PF( P < 0. 05); Western blot results showed that Caspase-3 and Cleave caspase3 expression increased with the increase of PF concentration( P < 0. 05),but Bcl-2 contrast with it. Conclusion: Paeoniflorin can inhibit the cell proliferation and promote cell apoptosis on human pancreatic cancer cell line Panc-1 cells,its mechanisms is to activate key enzyme Caspase-3 pathway.

关键词(KeyWords): 芍药苷;胰腺肿瘤;癌;Panc-1细胞;增殖;凋亡
paeoniflorin;pancreatic neoplasm;carcinoma;Panc-1 cells;proliferation;apoptosis

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(81672906);; 贵州省肝胆外科临床医学研究中心资助项目[黔科合平台人才(2017)5404];; 贵州省高层次创新人才培养计划“十”层次人才项目[黔科合平台人才(2016)5647];; 贵州省科学技术厅-贵阳医学院院士工作站肝胆外科分站项目[黔科合院士站(2015)4013];; 贵州省孙诚谊“肝胆胰脾疾病诊治”导师工作室项目[黔教研合GZS(2016)09];; 贵州省科学技术基金项目[黔科合J字(2015)2013号];; 贵州省科学技术厅-贵州医科大学附属医院联合基金项目[黔科合J字(2015)2013号];; 贵州省第四批人才基地基金资助项目[黔省专合字(2012)94号]

作者(Author): 黄巍;孙诚谊;喻超;潘耀振;田舍;张乙凡;陈世裕;江建新;
HUANG Wei;SUN Chengyi;YU Chao;PAN Yaozhen;TIAN She;ZHANG Yifan;CHEN Shiyu;JIANG Jianxin;Guizhou Medical University;Department of Hepatobiliary Surgery,the Affiliated Hospital of Guizhou Medical University;Renmin Hospital of Wuhan University;Hubei Provincial Institute of Digestive Diseases;

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DOI: 10.19367/j.cnki.1000-2707.2018.08.002

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