贵州医科大学学报

2015, v.40;No.173(02) 130-134+137

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幽门螺杆菌对小鼠胃黏膜G、D细胞及胃泌素和生长抑素的影响
Changes of Gastric Mucosal G,D Cells and m RNA Levels of Gastrin and Somatostatin in Helicobacter pylori-infected Mice

张岚;孙朝琴;张姝;张然;莫非;
ZHANG Lan;SUN Chaoqin;ZHANG Shu;ZHANG Ran;MO Fei;Department of Medical Laboratory,Guiyang Medical College;Department of Medical Laboratory,Liaocheng People's Hospital;

摘要(Abstract):

目的:研究幽门螺杆菌(Hp)感染对胃黏膜G、D细胞及胃泌素(GAS)、生长抑素(SS)表达的影响。方法:Hp标准菌SS2000菌液灌胃C57BL/6小鼠构建Hp感染小鼠慢性胃炎动物模型,免疫组化染色观察小鼠胃黏膜G、D细胞的改变,图像分析软件(Image-Pro-Plus,IPP)分析GAS、SS阳性灰度值,Real time-PCR检测小鼠胃窦黏膜GAS mRNA和SS mRNA相对表达量。结果:与空白组比较,模型组G细胞数目、GAS灰度值、GAS m RNA表达水平显著增高(P<0.05),D细胞数目、SS灰度值、SS mRNA表达水平无明显改变。结论:Hp感染可引起胃黏膜GAS mRNA表达上调,胃窦黏膜G细胞增多,促进GAS分泌,可能是胃黏膜病理改变加重的原因。
Objective: To investigate the influence of Helicobacter pylori( Hp) infection on gastric mucosal G cells and D cells,and the expression of gastrin( GAS) and somatostatin( SS) m RNA in mice. Methods: SPF C57 BL /6 mice were given Hp SS2000 by gavage to construct animal model of chronic gastritis. Adopting immunohistochemistry staning and image-pro-plus( IPP) to detect the expression and localization of GAS and SS in gastric antrum tissue,and analyzing the changes on G cell and D cell. The GAS and SS m RNA expression levels in gastric mucosa were detected before and after Helicobacter pylori infected by real time PCR. Results: Comparing with the blank group,model group's number of G cells,GAS gray value,GAS expression level of m RNA was significantly increased( P <0. 05); the number of D cells,SS gray value,SS m RNA expression level of model group did not significant changed( P > 0. 05). Conclusions: The infection of Hp can upregulate the expression of GAS m RNA in gastric mucosa,and increase the amount of G cells in gastric mucosa,and promote the secretion of GAS. It may be a cause of gastric mucosal pathological change aggravation.

关键词(KeyWords): 螺杆菌,幽门;G细胞;D细胞;胃泌素;生长抑素
Helicobacter pylori;G cell;D cell;gastrin;somatostatin

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基金项目(Foundation): 高等学校特色专业建设点[(2010)15];; 贵州省科技厅、贵阳医学院联合基金资助项目[黔科合LG(2012)054号];贵州省科技厅、贵阳医学院联合基金资助项目[黔科合LG(2012)012号];; 贵州省科技计划课题[黔科合J字(2014)2027号];; 贵阳医学院附院博士基金项目(2014)

作者(Author): 张岚;孙朝琴;张姝;张然;莫非;
ZHANG Lan;SUN Chaoqin;ZHANG Shu;ZHANG Ran;MO Fei;Department of Medical Laboratory,Guiyang Medical College;Department of Medical Laboratory,Liaocheng People's Hospital;

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