贵州医科大学学报

2010, (01) 17-21

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丹芪合剂和依那普利对糖尿病大鼠肾脏VEGF mRNA的影响
Effects of Danqi Mixture and Enalapril on Expression of VEGF mRNA in Kidney of Rats with Diabetic Nephropathy

王圆圆;刘瑞霞;肖瑛;石明隽;郭兵;
WANG Yuanyuan,LIU Ruixia,XIAO Ying,SHI Mingjun,GUO Bing(Department of Pathophysiology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

摘要(Abstract):

目的:探讨丹芪合剂和依那普利对糖尿病大鼠肾脏病理结构和血管内皮生长因子(VEGF)、转化生长因子-β1(TGF-β1)、纤维素连接蛋白(FN)表达的影响。方法:雄性SD大鼠24只随机分成4组,分别为正常对照组(C组)、糖尿病组(D组)、依那普利治疗组(E组)以及丹芪合剂治疗组(Y组)。尾静脉注射链脲菌素(STZ)复制糖尿病大鼠模型,12周处死大鼠,生化方法测血糖、血肌酐、胆固醇、甘油三脂及24h尿蛋白量,计算肾脏指数(KI);HE染色光镜观察肾组织形态;免疫组织化学法检测肾组织TGF-β1、FN的表达;RT-PCR检测肾组织VEGFmRNA表达情况。结果:与C组相比,糖尿病大鼠KI、血糖、血肌酐、胆固醇、甘油三脂、24h尿蛋白量均显著增高并出现蛋白尿,E组和Y组大鼠除血糖、KI外均显著低于D组。免疫组织化学结果显示正常肾组织中TGF-β1几乎没有阳性染色,FN有少量表达,而DN时,TGF-β1和FN表达都显著增加(P<0.01),E组和Y组大鼠,FN和TGF-β1表达下调(P<0.01)。RT-PCR结果提示,与C组相比,D组VEGFmRNA明显上调(P<0.05),而E组和Y组大鼠较D组表达减少。结论:依那普利和丹芪合剂对糖尿病大鼠肾脏具有保护作用,其机制可能与其下调肾组织中VEGFmRNA有关。
Objective:To investigate the effects of Danqi mixture and enalapril on the expression of vascular endothelial growth factor(VEGF),transforming growth factorβ1(TGF-β1),and fibronectin(FN),of rats with diabetic nephropathy.Methods:Twenty-four SD male rats were randomly divided into normal control group(group C),diabetic group(group D),diabetic enalapril-treated group(group E),and diabetic Danqi-treated group(group Y)with 6 rats in each.The diabetic rat model was produced with injection of streptozotocin.Blood glucose(BG),triglyceride,cholesterol,creatinine,and 24 h urine protein(UP)were analyzed with biochemical methods in 12 weeks and kidney indexes were analyzed as well.The pathomorphology of kidneys were examined with HE staining sections.Immunohistochemistry was employed to detect the expression of TGF-β1 and FN protein in renal tissue of all groups.The mRNA levels of VEGF in renal cortex were examined by using RT-PCR.Results:The levels of BG,triglyceride,cholesterol,creatinine,UP,and kidney indexes were higher remarkably in group D than those in group C.The parameters mentioned above except BG and KI in groups E and Y were notably lower than those in group D.Only a little FN protein expression and almost no expression of TGF-β1 could be seen in group C,while the expression of TGF-β1 and protein FN and VEGF mRNA content were significantly higher in group D than those in group C(P<0.01).In groups E and Y,the expression of TGF-β1 and the protein FN decreased compared with those in group D(P<0.01).Compared with that of group C,the expression of VEGF mRNA was remarkably higher in group D(P<0.05).In groups E and Y,the expression of the VEGF mRNA was lower than in group D.Conclusion:Danqi mixture and enalapril have protective effect to kidney of diabetic rats,which may relate to the down-regulation of VEGF expressions in nephridial tissues.

关键词(KeyWords): 糖尿病肾病;转化生长因子β;大鼠,Sprague-Dawley;血管内皮生长因子;丹芪合剂
diabetic nephropathies;transforming growth factor beta;rats,Sprague-Dawley;vascular endothelial growth factor;Danqi mixture

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基金项目(Foundation): 贵州省科技攻关资助项目(编号:黔科字S[2007]1033号);; 贵州省科技基金资助项目(编号:黔科合J字[2008]2167号)

作者(Author): 王圆圆;刘瑞霞;肖瑛;石明隽;郭兵;
WANG Yuanyuan,LIU Ruixia,XIAO Ying,SHI Mingjun,GUO Bing(Department of Pathophysiology,Guiyang Medical College,Guiyang 550004,Guizhou,China)

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