胡婷;罗鹏;
HU Ting;LUO Peng;The Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education Department of Toxicology,School of Public Health,Guizhou Medical University;

• 1:贵州医科大学公共卫生学院环境污染与疾病监控省部共建教育部重点实验室

摘要(Abstract)：

目的:探讨竹荪多糖对砷中毒大鼠体内砷含量的影响。方法:将24只清洁级健康SD大鼠均分为对照组、模型组、竹荪干预组3组,每组雌雄各半;对照组以常规饲料饲养,竹荪干预组和模型组喂饲砷含量50 mg/kg的饲料,竹荪干预组每日以10 g/L的竹荪多糖按大鼠体质量20 m L/kg灌胃,观察各组大鼠喂养过程中的毛发光泽度等一般情况,记录喂养0、30、60及90 d时大鼠的体质量;喂养3月时,取各组大鼠血液及尿液、并处死大鼠取肝脏组织,采用原子荧光光谱法检测其中砷含量,同时称取各组大鼠肝脏重量并计算脏器系数。结果:对照组大鼠发育正常,精神良好,毛发有光泽,模型组大鼠生长中等,体态中等,毛发较暗淡且稀疏,竹荪干预组大鼠发育、精神及毛发光泽度介于两组之间;与对照组相比,饲喂相同的时间,模型组和竹荪干预组体质量均降低,在饲喂30 d、90 d时差异有统计学意义(P<0.05);开始饲喂后,竹荪干预组大鼠体质量与模型组相比升高,但仅30 d时差异有统计学意义(P<0.05);与对照组相比,模型组和竹荪干预组脏器重量和脏器系数、肝砷、血砷、尿砷均升高(P<0.05);与模型组相比,竹荪干预组与模型组相比,竹荪干预组肝砷降低而尿砷升高(P<0.05)。结论:竹荪多糖可以降低砷中毒大鼠肝脏、血液、尿液中的砷含量。
Objective: To investigate the effect of dictyophora polysaccharide on arsenic content in arsenic poisoning rats. Methods: A total of 24 healthy adult clean SD rats were randomly divided into control group,dictyophora polysaccharide intervention group and model group,half male and half female in each group. The control received ordinary feed. Dictyophora polysaccharide intervention group and the model group received feed of 50 mg / kg arsenic content. Meanwhile,dictyophora polysaccharide intervention group received intragastric administration of dictyophora polysaccharide(10 g / L) at20 m L / kg every day. The rats' hair gloss and other general conditions in the feeding process were observed and the rats' body weight at 0,30,60 and 90 d after feeding were recorded. 3 months after feeding and treatment,the blood and urine of rats in each group were taken,and the rats were sacrificed to get the liver tissue. The content of arsenic was detected by atomic fluorescence spectrometry in rats' blood,urine and liver tissue,and the liver weight of rats in each group was calculated and the organ coefficient was calculated. Results: The rats in the control group developed normally,the spirit was good,and the hair was glossy while the rats in model group developed relatively medium,and the hair was dark and sparse. In dictyophora polysaccharide intervention group,the rats ' development,spirit and hair gloss were between control group and model group. Compared with control group,at the same time of development,the body weight of rats in model group and dictyophora polysaccharide intervention group decreased,and the differences at 30 d,90 d after feeding were statistically significant(P < 0. 05). After feeding,in dictyophora polysaccharide intervention group rats' body weight increased compared with that of model group,but there was significant difference at 30 d after feeding(P < 0. 05). Compared with control group,in model group and dictyophora polysaccharide intervention group the liver weight,organ coefficient,arsenic in liver,arsenic in blood,and arsenic in urine were increased(P < 0. 05). Compared with model group,in dictyophora polysaccharide intervention group arsenic in liver decreased while arsenic in urine increased(P < 0. 05). Conclusion: Dictyophora polysaccharide can reduce the arsenic content in the liver,blood and urine in arsenic poisoning rats.

关键词(KeyWords)： 竹荪多糖;亚砷酸钠;砷中毒;肝损伤;模型,动物;大鼠,Sprague Dawley
dictyophora polysaccharide;sodium arsenite;arsenic poisoning;liver injury;animal model;rats,Sprague-Dawley

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81160336);; 贵州省科技厅农业攻关计划项目[黔科合NY字(2011)3055号];贵州省科技厅[黔科合J字(2010)2188];; 贵州省教育厅基金项目[黔省专合字(2010)39号]

作者(Author): 胡婷;罗鹏;
HU Ting;LUO Peng;The Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education Department of Toxicology,School of Public Health,Guizhou Medical University;

Email:

DOI: 10.19367/j.cnki.1000-2707.2016.11.004

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