贵州医科大学学报

2000, (02) 116-118

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体外溶血对血钾测定的影响及校正
The Influence of Hemolysis in Vitro on the Determination of Plasma Potassium and Its Correction

陶建蜀,黄海
Tao Jianshu, Huang Hai Department Clinical Laboratory of Guiyang Medical College

摘要(Abstract):

应用三波长分光光度法直接测定血浆血红蛋白 (FHb) ,以此作为溶血程度的判定指标 ,探讨溶血对血钾测定的影响。测定结果表明 :血浆FHb每增高 1g ,血钾值增高约 12 %。FHb与血钾增高值 ([K+ ]溶 - [K+ ]未)之间有较好的相关性 (R =0 938,P <0 0 1)。由此导出校正公式 :[K+ ]未=[K+ ]溶 - 0 513FHb +0 0 92 ,校正值平均相对误差 4 7% ;对 5例临床溶血标本进行校正 ,平均相对误差为 4 1%。
Free hemoglobin (FHb)in plasma had directly been determined with three-wavelength spectrophotometedry as estimating index of hemolysis degree in order to know the influence of hemolysis on plasma potassium.The results showed that the leve of potassium in plasma rised by 12% as the plasma FHb increased one gram. Between FHb and the rised value of plasma potassium ([K +] 溶-[K +] 未),there was good relativity (R=0 938,P<0 01). A corrective formula might be guided as ( [K +] 未=[K +] 溶-0 513 FHb+0 092),and the average relative error after correction was 4.7%. Usign this formula to correct the test value of plasma potassium at 5 hemolyed samples,the average relative error after correction was 4.1%.

关键词(KeyWords): 溶血;钾;血红蛋白类
hemolysis; potassium; hemoglobins

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作者(Author): 陶建蜀,黄海
Tao Jianshu, Huang Hai Department Clinical Laboratory of Guiyang Medical College

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DOI: 10.19367/j.cnki.1000-2707.2000.02.003

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