贵州医科大学学报

2019, v.44;No.228(09) 1034-1038

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钙卫蛋白S100A8/A9对口腔鳞癌细胞增殖和迁移的影响
Effects of Calprotectin(S100A8/A9)on Proliferation and Migration of Oral Squamous Carcinoma Cells

鲍玲娜;马洪;李超;
BAO Lingna;MA Hong;LI Chao;Department of Oral and Maxillofacial Surgery, Affiliated Stomatology Hospital of Guizhou Medical University;Department of Head and Neck Surgery, Sichuan Cancer Hospital& Institute, Sichuan Cancer Prevention and Treatment Center;

摘要(Abstract):

目的:研究钙卫蛋白S100A8/A9对口腔鳞癌(OSCC)细胞SCC-25增殖和迁移能力的影响。方法:选取人舌鳞状细胞癌细胞SCC-25作为模型细胞,将S100A8和S100A9人重组蛋白以质量比1∶1均匀混合形成钙卫蛋白S100A8/A9,分别以0 mg/L(对照组)、5、10、20、30和40 mg/L的浓度作用于SCC-25细胞24及48 h,观察钙卫蛋白S100A8/A9对SCC-25细胞增殖能力的影响;选取5 mg/L钙卫蛋白S100A8/A9处理SCC-25细胞,采用Transwell小室及细胞划痕实验观察S100A8/A9对SCC-25细胞的侵袭及迁移能力的影响。结果:5、10、20、30和40 mg/L钙卫蛋白S100A8/A9作用下SCC-25细胞24 h时的增殖能力较对照组增强(P<0.05),5、10、20和30 mg/L钙卫蛋白S100A8/A9作用下SCC-25细胞48 h时的增殖能力表现出相同趋势,但40 mg/L钙卫蛋白S100A8/A9作用SCC-25细胞48 h时的增殖能力较对照组减弱(P<0.05);Transwell小室实验显示,5 mg/L钙卫蛋白S100A8/A9作用的实验组穿过聚碳酸酯膜的SCC-25细胞数目多于对照组,差异有统计学意义(P<0.05);划痕实验结果显示,5 mg/L钙卫蛋白S100A8/A9作用的实验组在24、48及72 h后细胞划痕面积明显小于对照组。结论:5 mg/L钙卫蛋白S100A8/A9促进SCC-25细胞的增殖和迁移。
Objective: To investigate the effects of calprotectin(S100A8/A9) on the proliferation and migration of oral squamous carcinoma(OSCC) cell line SCC-25. Methods: Squamous carcinoma cell SCC-25 from human tongues as model cell. Human recombinant protein S100A8 and S100A9 were homogeneously mixed to form calprotectin S100A8/A9 with mass ration 1 ∶1; S100A8/A9 as 0 mg/L(control group), concentration of 5 mg/L,10 mg/L,20 mg/L,30 mg/L and 40 mg/L applied to SCC-25 cells for 24 h and 48 h, observing effect of calprotectin S100A8/A9 on SCC-25 proliferation. Selecting SCC-25 cells were treated with 5 mg/L S100A8/A9 protein,the invasion and migration ability of SCC-25 cells was observed by Transwell chamber assay and wound healing assay. Results: At 24 h,the proliferation of SCC-25 cells was enhanced by exogenous S100A8/A9 protein at 5 mg/L,10 mg/L,20 mg/L,30 mg/L and 40 mg/L compared with the control group(P<0.05);at 48 h,SCC-25 cells showed the same trend at 5 mg/L,10 mg/L,20 mg/L,30 mg/L and 40 mg/L; but at 40 mg/L,the proliferation was weakened compared with the control group(P<0.05). Transwell chamber showed that SCC-25 cells cross the polycarbonate membrane enhanced by 5 mg/L calprotectin S100A8/A9 were more than control group, differences were statistically significant(P<0.05). Wound healing assay showed that the wound area of the experimental group treated with 5 mg/L calprotectin S100A8/A9 was significantly smaller than that in the control group after 24 h, 48 h and 72 h. Conclusion: 5 mg/L calprotectin S100A8/A9 can promote the proliferation and migration of SCC-25 cells.

关键词(KeyWords): 口腔鳞状细胞癌;细胞增殖;细胞迁移分析;细胞运动;钙卫蛋白
oral squamous carcinoma;cell proliferation;cell migration assays;cell movement;calprotectin

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基金项目(Foundation): 贵州省科技厅国际合作计划[黔科合外G字(2014)7009]

作者(Author): 鲍玲娜;马洪;李超;
BAO Lingna;MA Hong;LI Chao;Department of Oral and Maxillofacial Surgery, Affiliated Stomatology Hospital of Guizhou Medical University;Department of Head and Neck Surgery, Sichuan Cancer Hospital& Institute, Sichuan Cancer Prevention and Treatment Center;

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DOI: 10.19367/j.cnki.1000-2707.2019.09.009

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